Nephrin Antikörper (AA 1100-1150)

Produktnummer ABIN6992074

Produktdetails anti-Nephrin Antikörper

Target: Nephrin (NPHS1) (Nephrosis 1, Congenital, Finnish Type (Nephrin) (NPHS1))

Bindungsspezifität: AA 1100-1150

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Nephrin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Aufreinigung: Nephrin antibody is affinity chromatography purified via peptide column.

Immunogen: Anti-Nephrin antibody was raised against a peptide corresponding to 15 amino acids near the center of human Nephrin. The immunogen is located within amino acids 1100-1150 of Nephrin.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1-2 μ,g/mL, IHC: 5 μ,g/mL, IF: 20 μ,g/mL.

Antibody validated: Western Blot in human and mouse samples, Immunohistochemistry in human samples, Immunofluorescence in human samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Nephrin antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Nephrin antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Details zu Nephrin

Target: Nephrin (NPHS1) (Nephrosis 1, Congenital, Finnish Type (Nephrin) (NPHS1))

Andere Bezeichnung: Nephrin (NPHS1 Produkte)

Synonyme: NPHS1 antikoerper, nphs1l antikoerper, nphs1 antikoerper, CNF antikoerper, NPHN antikoerper, nephrin antikoerper, NephrinB antikoerper, NPHS1, nephrin antikoerper, nephrosis 1, congenital, Finnish type (nephrin) antikoerper, NPHS1 nephrin S homeolog antikoerper, nephrin antikoerper, NPHS1 nephrin antikoerper, nephrosis 1, nephrin antikoerper, NPHS1 antikoerper, nphs1 antikoerper, nphs1.S antikoerper, LOC5579127 antikoerper, Nphs1 antikoerper

Hintergrund: Nephrin is strongly expressed in renal glomeruli and is a member of the immunoglobulin family of cell adhesion molecules. Mutations in the Nephrin gene result in congenital nephrotic syndrome, an autosomal-recessive disorder characterized by massive proteinuria in utero and nephrosis at birth (1). Renal glomeruli allow normal kidneys to filter plasma so that it is very pure. Nephrin is expressed in the podocyte slit-diaphragm of the renal glomeruli in a manner that suggests that Nephrin molecules homodimerize in an anti-parallel fashion similar to cadherin interactions in adherens junctions. Thus, Nephrin may constitute the entire extracellular structure of the slit-diaphragm (2,3).

Molekulargewicht: Predicted: 125, 137 kDa

Observed: 125 kDa

Gen-ID: 4648

NCBI Accession: NP_004637

UniProt: O60500

Pathways: Regulation of Actin Filament Polymerization, Skeletal Muscle Fiber Development