ELISA, Western Blotting (WB), Immunofluorescence (IF)
Spezifität
CRIM1 antibody is human reactive. CRIM1 antibody is predicted to not cross-react with CRIM2.
Aufreinigung
CRIM1 Antibody is affinity chromatography purified via peptide column.
Immunogen
CRIM1 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human Crim1. The immunogen is located within amino acids 910 - 960 of CRIM1.
CRIM1
Reaktivität: Human
Wirt: Kaninchen
Polyclonal
Alexa Fluor 680
Applikationshinweise
CRIM1 antibody can be used for detection of Crim1 by Western blot at 1 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
1 mg/mL
Buffer
CRIM1 Antibody is supplied in PBS containing 0.02 % sodium azide.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
-20 °C,4 °C
Informationen zur Lagerung
CRIM1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CRIM1 Antibody: CRIM1 (cysteine-rich motor neuron 1), a glycosylated type I transmembrane protein, plays a role in tissue development i.e. capillary formation and maintenance during angiogenesis. It contains an N-terminal IGF-binding protein-like motif and six von Willebrand-like cysteine-rich repeats (CRRs) in its extracellular domain. CRIM1 interacts with BMP4 and BMP7 via the CRRs and functions as an antagonist. CRIM1 is developmentally expressed in a number of tissues including the pancreas, kidney, placenta, brain and blood vessels. CRIM1 may participate in CNS and placental development by interacting with growth factors involved in motor neuron differentiation and survival.