PLEKHM1 Antikörper (N-Term)

Produktnummer ABIN6991224

Produktdetails anti-PLEKHM1 Antikörper

Target: PLEKHM1 (Pleckstrin Homology Domain Containing, Family M (With RUN Domain) Member 1 (PLEKHM1))

Bindungsspezifität: AA 50-100, N-Term

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PLEKHM1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Aufreinigung: PLEKHM1 Antibody is affinity chromatography purified via peptide column.

Immunogen: PLEKHM1 antibody was raised against an 18 amino acid synthetic peptide from near the amino terminus of human PLEKHM1. The immunogen is located within amino acids 50 - 100 of PLEKHM1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: PLEKHM1 antibody can be used for detection of PLEKHM1 by Western blot at 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PLEKHM1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: PLEKHM1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Details zu PLEKHM1

Target: PLEKHM1 (Pleckstrin Homology Domain Containing, Family M (With RUN Domain) Member 1 (PLEKHM1))

Andere Bezeichnung: PLEKHM1 (PLEKHM1 Produkte)

Synonyme: AP162 antikoerper, B2 antikoerper, OPTB6 antikoerper, BC038943 antikoerper, D330036J23Rik antikoerper, pleckstrin homology and RUN domain containing M1 antikoerper, pleckstrin homology domain containing, family M (with RUN domain) member 1 antikoerper, PLEKHM1 antikoerper, Plekhm1 antikoerper

Hintergrund: PLEKHM1 Antibody: PLEKHM1 is a member of the M family of Pleckstrin homolog domain-containing proteins, a group of proteins containing a RUN domain, two pleckstrin homology domains, and a cysteine-rich domain. It was identified through segregation analysis as a cause of osteopetrosis in humans. PLEKHM1 co-localizes with Rab7 to late endosomal/lysosomal vesicles in HEK293 and osteoclast-like cells, with this co-localization dependent on the prenylation of Rab7. Monocytes from a patient homozygous for a mutated form of PLEKHM1differentiated into osteoclasts normally, but failed to form ruffled borders and showed little evidence of bone resorbtion when cultured on dentine discs. Another mutation of PLEKHM1 impaired vesicular acidification and increased TRACP secretion in osteoclasts, suggesting that PLEKHM1 has critical roles in endosomal maturation and may be important in osteoclast-osteoblast cross-talk.

Gen-ID: 9842

UniProt: Q9Y4G2