Piwil/ Mili (AA 885-973) Antikörper (Alexa Fluor 750)

Produktnummer ABIN6982409

Produktdetails

Target: Piwil/ Mili

Bindungsspezifität: AA 885-973

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Alexa Fluor 750

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Dog,Cow,Pig,Rabbit,Guinea Pig

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Piwil/Mili

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: Piwil/ Mili

Andere Bezeichnung: Piwil/Mili

Hintergrund:

Synonyms: HILI, Hiwi like, MILI, Miwi like, Piwi like 2, Piwi-like 2, Piwil 2, Piwil-2, Piwil2, Cancer/testis antigen 80, CT80, PIWL2_HUMAN, Piwil 1, Piwil 3, Piwil1, Piwil3, Piwi-like protein 1, PL2L60

Background: HILI is a 973 amino acid protein encoded by the human gene PIWIL2. HILI belongs to the argonaute family and contains one PAZ domain and one PIWI domain. HILI is a cytoplasmic protein that is expressed in adult testis and in most tumors. It regulates spermatogenesis and primordial germ cell production and has an essential role in meiotic differentiation of spermatocytes and in self-renewal of spermatogonial stem cells. Expression of HILI can modulate expression of genes involved in stem cell proliferation (such as PDGFR-b), in energy metabolism (such as Glut1), in cell-cell interaction (such as Integrin a6, GJA7, THY-1 and CD9), and in germ cell differentiation (such as STRA8). It may also play a role as a regulatory factor of Stat3/Bcl-xS/L/CCND1 pathway. Repression of HILI can inhibit tumor cell growth. HILI acts as an oncogene by inhibition of apoptosis and promotion of proliferation in tumors.

Gen-ID: 55124