PUF60 Antikörper (AA 1-100) (Alexa Fluor 488)

Produktnummer ABIN6977705

Produktdetails anti-PUF60 Antikörper (Alexa Fluor 488)

Target: PUF60 (Poly-U Binding Splicing Factor 60KDa (PUF60))

Bindungsspezifität: AA 1-100

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PUF60 Antikörper ist konjugiert mit Alexa Fluor 488

Applikation: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PUF60

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu PUF60

Target: PUF60 (Poly-U Binding Splicing Factor 60KDa (PUF60))

Andere Bezeichnung: PUF60 (PUF60 Produkte)

Synonyme: FIR antikoerper, RoBPI antikoerper, SIAHBP1 antikoerper, fc21a05 antikoerper, wu:fc21a05 antikoerper, wu:fi43a08 antikoerper, 2410104I19Rik antikoerper, 2810454F19Rik antikoerper, Siahbp1 antikoerper, fe37c05 antikoerper, repressor antikoerper, si:ch211-12p8.2 antikoerper, si:zc12p8.2 antikoerper, wu:fb33e11 antikoerper, wu:fe37c05 antikoerper, zgc:86806 antikoerper, poly(U) binding splicing factor 60 antikoerper, poly-U binding splicing factor a antikoerper, poly-U binding splicing factor 60 antikoerper, poly-U binding splicing factor b antikoerper, PUF60 antikoerper, puf60a antikoerper, Puf60 antikoerper, puf60b antikoerper

Hintergrund:

Synonyms: 60 kDa poly(U)-binding-splicing factor, FBP-interacting repressor, FIR, FLJ31379, FUSE-binding protein-interacting repressor, Poly U binding splicing factor 60KDa, Poly(U)-binding-splicing factor PUF60, poly-U binding splicing factor PUF60, PUF60, PUF60_HUMAN, pyrimidine tract binding splicing factor, Ro ribonucleoprotein-binding protein 1, Ro-binding protein 1, RoBP1, RoBPI, siah binding protein 1, Siah-binding protein 1, Siah-BP1, SIAHBP1

Background: DNA- and RNA-binding protein, involved in several nuclear processes such as pre-mRNA splicing, apoptosis and transcription regulation. In association with FUBP1 regulates MYC transcription at the P2 promoter through the core-TFIIH basal transcription factor. Acts as a transcriptional repressor through the core-TFIIH basal transcription factor. Represses FUBP1-induced transcriptional activation but not basal transcription. Decreases ERCC3 helicase activity. Does not repress TFIIH-mediated transcription in xeroderma pigmentosum complementation group B (XPB) cells. Is also involved in pre-mRNA splicing. Promotes splicing of an intron with weak 3'-splice site and pyrimidine tract in a cooperative manner with U2AF2. Involved in apoptosis induction when overexpressed in HeLa cells. Isoform 6 failed to repress MYC transcription and inhibited FIR-induced apoptosis in colorectal cancer. Isoform 6 may contribute to tumor progression by enabling increased MYC expression and greater resistance to apoptosis in tumors than in normal cells. Modulates alternative splicing of several mRNAs. Binds to relaxed DNA of active promoter regions. Binds to the pyrimidine tract and 3'-splice site regions of pre-mRNA, binding is enhanced in presence of U2AF2. Binds to Y5 RNA in association with TROVE2. Binds to poly(U) RNA.

Gen-ID: 22627

UniProt: Q9UHX1