PRPF8 Antikörper

Produktnummer ABIN6944681

Produktdetails anti-PRPF8 Antikörper

Target: PRPF8 (PRP8 Pre-mRNA Processing Factor 8 (PRPF8))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser PRPF8 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Purified by Protein A.

Immunogen: Recombinant protein within 1200 of human PRPF8.

Klon: 1A7

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu PRPF8

Target: PRPF8 (PRP8 Pre-mRNA Processing Factor 8 (PRPF8))

Andere Bezeichnung: PRPF8 (PRPF8 Produkte)

Synonyme: id:ibd1257 antikoerper, ik:tdsubc_2a9 antikoerper, im:7141966 antikoerper, tdsubc_2a9 antikoerper, wu:fb37c02 antikoerper, wu:fb73e06 antikoerper, xx:tdsubc_2a9 antikoerper, zgc:56504 antikoerper, hprp8 antikoerper, prp-8 antikoerper, prp8 antikoerper, prpc8 antikoerper, rp13 antikoerper, HPRP8 antikoerper, PRP8 antikoerper, PRPC8 antikoerper, RP13 antikoerper, SNRNP220 antikoerper, AU019467 antikoerper, D11Bwg0410e antikoerper, DBF3/PRP8 antikoerper, Prp8 antikoerper, Sfprp8l antikoerper, pre-mRNA processing factor 8 antikoerper, pre-mRNA processing factor 8 S homeolog antikoerper, Prpf8 antikoerper, prpf8 antikoerper, prpf8.S antikoerper, PRPF8 antikoerper

Hintergrund:

Synonyms: Pre-mRNA-processing-splicing factor 8, 220 kDa U5 snRNP-specific protein, PRP8 homolog, Splicing factor Prp8, p220, PRPF8, PRPC8

Background: PRP8, also designated pre-mRNA-processing-splicing factor 8, is a highly conserved nuclear protein and a central component of the catalytic core of the spliceosome, where it may be involved in various molecular rearrangements. PRP8, which is widely expressed, plays a role in transesterification reactions that regulate splicesome-induced pre-mRNA splicing. Specifically, PRP8 interacts with the GU dinucleotide at the 5' splice site (5'SS) and forms a specific UV-inducible cross-link. It also interacts functionally with the 3'SS, affecting the efficiency of the second catalytic step. PRP8 may play a role in the first transesterification step, as PRP8 mutations that prohibit negative regulation of PRP28 or PRP44/Brr2 subsequently block U4 activation. In addition, PRP8 interacts with a conserved region of U6 that is instrumental in the formation of the catalytic core of the spliceosome.

Gen-ID: 10594

UniProt: Q6P2Q9