HPV18 Antikörper

Produktnummer ABIN6937433

Produktdetails anti-HPV18 Antikörper

Target: HPV18 (HPV-18) (Human Papilloma Virus Type 18 (HPV-18))

Reaktivität: Human Papillomavirus 18 (HPV-18)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HPV18 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Staining Methods (StM)

Aufreinigung: Purified by Protein A/G

Immunogen: HPV18 E6-β-galactosidase fusion protein

Klon: HPV18-1297

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: HPV-18 infected cells. Cervical tissue.

Known Application: Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 10 mM PBS without BSA and without Azide.

Konservierungsmittel: Azide free

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months.

Haltbarkeit: 24 months

Details zu HPV18

Target: HPV18 (HPV-18) (Human Papilloma Virus Type 18 (HPV-18))

Andere Bezeichnung: HPV-18 (Human Papilloma Virus 18) (HPV-18 Produkte)

Substanzklasse: Viral Protein

Hintergrund: Human papilloma viruses (HPVs) can be classified as either high risk or low risk according to their association with cancer. HPV16 and HPV18 are the most common of the high risk group while HPV6 and HPV11 are among the low risk types. Approximately 90 % of cervical cancers contain HPV DNA of the high risk types. Mutational analysis has shown that the E6 and E7 genes of the high risk HPVs are necessary and sufficient for HPV transforming function. The specific interactions of the E6 and E7 proteins with p53 and pRB, respectively, correlate with HPV high and low risk classifications. The high risk HPV E7 proteins bind to pRB with a higher affinity than do the low risk HPV proteins, and only the high risk HPV E6 proteins form detectable complexes with p53 in vitro.

Molekulargewicht: 16kDa