CYP1A1/2 Antikörper

Produktnummer ABIN6937070

Produktdetails anti-CYP1A1/2 Antikörper

Target: CYP1A1/2 (Cytochrome P450, Family 1, Subfamily A, Polypeptide 1/2 (CYP1A1/2))

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CYP1A1/2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Spezifität: Cytochrome P450 oxidase (commonly abbreviated CYP) is a generic term for a large number of related, but distinct, oxidative enzymes important in vertebrate physiology. The cytochrome P450 mixed-function monooxygenase system is probably the most important element of Phase I metabolism in mammals. P450s are membrane-bound, either in the inner membrane of mitochondria or in the endoplasmic reticulum of cells where they metabolise thousands of endogenous and exogenous compounds. In the liver, these substrates include toxins, drugs, and other unneeded and potentially harmful molecules. Humans have 18 families of cytochrome P450 genes and 43 subfamilies, the CYP1 family is involved in drug metabolism and includes 3 subfamilies, 3 genes and 1 pseudogene.

Kreuzreaktivität (Details): Human. Mouse. Rat. Primate.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: 3-methylcholanthrene induced rat cytochrome P450 protein.

Klon: MC1

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HeLa cells. Liver or Cardiac muscle.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu CYP1A1/2

Target: CYP1A1/2 (Cytochrome P450, Family 1, Subfamily A, Polypeptide 1/2 (CYP1A1/2))

Andere Bezeichnung: CYP1A1 / CYP1A2 (CYP1A1/2 Produkte)

Synonyme: CP12 antikoerper, Cyp1a1 antikoerper, P450-3 antikoerper, cytochrome P450, family 1, subfamily a, polypeptide 2 antikoerper, Cyp1a2 antikoerper

Hintergrund: Aryl hydrocarbon hydroxylase, CP11, CP12, CYP1A1, CYP1A2, CYPIA1, CYPIA2, Cytochrome P450 family 1 subfamily A polypeptide 1, Cytochrome P450 family 1 subfamily A polypeptide 2, P1 450, P3 450, P450 form 4, P450 form 6, P450 P1, P450 P3,Cytochrome P450 1A1/1A2
Cellular localisation: Cytoplasmic

Molekulargewicht: 58kDa

Gen-ID: 1543, 1544, 437060

UniProt: P04798, P05177