MAP2K1 Antikörper (Mitogen-Activated Protein Kinase Kinase 1) (AA 2-35)

Details for Product anti-MAP2K1 Antibody No. ABIN686482
Antigen
  • Mek1
  • CFC3
  • MAPKK1
  • MEK1
  • MKK1
  • PRKMK1
  • MEKK1
  • Prkmk1
  • mek-2
  • si:ch211-242m18.2
  • wu:fj56a12
  • wu:fj61b01
  • zgc:56557
  • ATMEK1
  • F20B18.180
  • F20B18_180
  • MAP kinase/ ERK kinase 1
  • MITOGEN ACTIVATED PROTEIN KINASE KINASE
  • MITOGEN ACTIVATED PROTEIN KINASE KINASE 1
  • NMAPKK
  • MEK
  • mitogen activated protein kinase kinase 1
  • mitogen-activated protein kinase kinase 1
  • mitogen-activated protein kinase kinase 1 L homeolog
  • MAP kinase/ ERK kinase 1
  • Map2k1
  • MAP2K1
  • map2k1.L
  • map2k1
  • MEK1
Epitop
AA 2-35
59
47
45
37
34
33
31
28
25
24
24
18
15
15
15
14
10
10
9
9
9
7
6
5
5
5
4
4
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte (Rattus)
742
489
435
107
79
50
30
21
19
12
10
8
6
4
4
1
1
1
1
1
Wirt
Kaninchen
634
115
5
2
1
1
Klonalität
Polyklonal
Konjugat
Dieser MAP2K1 Antikörper ist unkonjugiert
31
20
18
17
17
17
17
17
17
10
9
9
5
5
5
5
5
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
547
298
209
123
120
68
29
19
18
10
9
8
8
6
4
2
1
1
Optionen
'Independent Validation' Siegel
Chargennummer 90915
Validierte Anwendung Immunohistochemistry
Positivkontrolle Human placenta
Negativkontrolle Human breast adipocytes
Bewertung Signal detected in positive control sample and not in negative control sample.
Primärantikörper
  • Antigen: Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1)
  • Catalog number: ABIN686482
  • Lot number: 90915
Isotypkontrolle
  • Antibody: Rabbit IgG isotype control
  • Lot number: C11245
Sekundärantikörper
  • Antibody: Biotinylated goat anti-rabbit/anti-mouse (Kit)
  • Lot number: D07640BA
Kontrollen
  • Positive control: Human placenta tissue stained with antibody
  • Negative control: Human breast adipocytes tissue stained with antibody
  • Isotype control: Human placenta tissue stained with isotype control
  • Secondary only control: Human placenta tissue stained with secondary antibody only
Protokoll
  • Immunohistochemistry was performed on a Ventana NEXes automated platform; instrument manufacturer specific reagents are italicized.
  • 1. Slides were preheated in convection oven at 60°C for 30 min
  • 2. Deparaffinization procedure: - 3 changes of Xylene, 5 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of 95% Ethanol, 3 min each - Rinsed in distilled water, 3 changes
  • 3. Heat retrieval procedure - Slides retrieved in 10.0 mM Citrate, pH6.0 in a 1000W microwave oven (~100°C) for 15 min. - Slides were allowed to cool (in citrate) for 30 min. - Slides were washed x 3 in Distilled water
  • 4. NEXes instrument procedure, iView DAB paraffin protocol (*abridged*): - Slide chamber warmed to 37°C
  • 5. Slides rinsed with *reaction buffer* x3
  • 6. *iView Inhibitor (H2O2)* applied and incubated for 4 min
  • 7. Slides rinsed with *reaction buffer*
  • 8. Antibody Application - Primary antibody diluted 1:250 in PBS (100 microliter applied/slide) - Ventana Isotype control applied neat - Slides Incubated overnight at room temperature (~12 hours ~25°C)
  • 9. Slides rinsed with *reaction buffer* x3
  • 10. *iView Biotinylated IgG* applied and incubated for 8 min
  • 11. Slides rinsed with *reaction buffer*
  • 14. *iView Streptavidin-Horseradish Peroxidase* applied and incubated for 8 min
  • 15. Slides rinsed with *reaction buffer*
  • 16. *iView DAB/H2O2* applied and incubated for 8 min
  • 17. Slides rinsed with *reaction buffer*
  • 18. *iView Copper* applied and incubated for 4 min
  • 19. Slides rinsed with *reaction buffer*
  • 20. Slides washed in Dawn Detergent/tap water
  • 21. Counterstain Procedure - Hematoxylin (Leica 560 MX) 30 sec - Slides washed in tap water, 1 min - Decolorized (10% Acetic Acid in 70% ethanol), 1 min - Slides washed in tap water, 1 min - Bluing (Austin Clear Ammonia), 1 min - Slides washed in tap water, 1 min
  • 22. Dehydration/coverslipping procedure: - 3 changes of 95% Ethanol, 3 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of Xylene, 5 min each - Mounted with Permount
  • 23. Imaging: Leica SCN 400F Whole Slide Scanner with Digital Image Hub and Leica Slidepath software
Anmerkungen
  • Deviations from protocol/procedure supplied by manufacturer:
  • Step 1: Heated tissue 60°C for 30 minutes; manufacturer heats for 45 minutes.
  • Step 2: No ethanol wash was performed during deparaffinization; manufacturer includes 1 wash of 80% ethanol for 3 minutes.
  • Step 3.1: Slides were heated for 15 minutes; manufacturer provides a range of 15-20 minutes.
  • Step 3.2: Slides were cooled for 30 minutes; manufacturer cools for 20 minutes.
  • Step 4: Italicized reagents and incubation time are fixed instrument parameters.
  • Step 5: Secondary species-specific serum block not used; manufacturer blocks with 5% normal goat serum for 2 hours.
  • Step 8.1: Antibody diluted in PBS at 1:250; manufacture did not recommend diluent or dilution.
  • Step 8.2.1: Primary antibody incubated at room temperature overnight; manufacturer incubates overnight 4°C with agitation.
Validierungsbilder
Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 2-35) antibody (ABIN686482) Figure 1: Human placenta tissue stained with anti-MAP2K1 (brown) and counterstained w...
Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 2-35) antibody (ABIN686482) Figure 2: Human breast adipose tissue stained with anti-MAP2K1 (brown) and countersta...
Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 2-35) antibody (ABIN686482) Figure 3: Human placenta tissue stained with isotype control antibody (brown) and cou...
Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 2-35) antibody (ABIN686482) Figure 4: Human placenta tissue stained with secondary antibody only (brown) and coun...
Immunogen KLH conjugated synthetic peptide derived from human MEK1
Isotyp IgG
Reinigung Purified by Protein A.
Plasmids, Primers & others Plasmide, Primers & weitere MAP2K1 products on genomics-online (e.g. as negative or positive controls)
Antigen
Andere Bezeichnung Mek1 (MAP2K1 Antibody Abstract)
Hintergrund

Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator-activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis.

Synonyms: CFC3, MEK1, MKK1, MAPKK1, PRKMK1, Dual specificity mitogen-activated protein kinase kinase 1, MAP kinase kinase 1, MAPKK 1, ERK activator kinase 1, MAPK/ERK kinase 1, MEK 1, MAP2K1

Gen-ID 5604
UniProt Q02750
Pathways MAPK Signalweg, RTK Signalweg, Interferon-gamma Pathway, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagie, Signaling of Hepatocyte Growth Factor Receptor
Applikations-hinweise WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20°C
Haltbarkeit 12 months
Bilder des Herstellers
Image no. 1 for anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 2-35) antibody (ABIN686482) Images provided the Independent Validation Program (badge number 029646)Formalin-fixe...
Image no. 2 for anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 2-35) antibody (ABIN686482) Paraformaldehyde-fixed, paraffin embedded human colon cancer, Antigen retrieval by bo...
Image no. 3 for anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 2-35) antibody (ABIN686482) Image provided by the Independent Validation Program (badge number 29760). Lane 1: He...
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