PLEKHH2 Antikörper (AA 1043-1092)
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- Target Alle PLEKHH2 Produkte
- PLEKHH2 (Pleckstrin Homology Domain Containing, Family H (With MyTH4 Domain) Member 2 (PLEKHH2))
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Bindungsspezifität
- AA 1043-1092
- Reaktivität
- Human, Maus, Rind (Kuh), Hund, Meerschweinchen, Pferd, Kaninchen, Fledermaus, Affe, Schwein
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser PLEKHH2 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Marke
- IHC-plus™
- Spezifität
- Human PLEKHH2
- Homologie
- Percent identity by BLAST analysis: Dog, Bovine, Rabbit, Horse, Guinea pig (100%) Rat (92%) Chicken (86%).
- Aufreinigung
- Immunoaffinity purified
- Immunogen
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Synthetic peptide located between aa1043-1092 of human PLEKHH2 (Q8IVE3, NP_742066). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig, Zebra finch (100%), Rat, Opossum (92%), Turkey, Chicken (86%), Xenopus, Drosophila (84%).
Type of Immunogen: Synthetic peptide - Isotyp
- IgG
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- Applikationshinweise
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Approved: IHC, IHC-P (10 μg/mL), WB
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. - Kommentare
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Target Species of Antibody: Human
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Distilled Water.
- Konzentration
- Lot specific
- Buffer
- Lyophilized from PBS with 2 % sucrose
- Handhabung
- Avoid repeat freeze-thaw cycles.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- Target
- PLEKHH2 (Pleckstrin Homology Domain Containing, Family H (With MyTH4 Domain) Member 2 (PLEKHH2))
- Andere Bezeichnung
- PLEKHH2 (PLEKHH2 Produkte)
- Synonyme
- RGD1304935 antikoerper, si:ch73-105b23.3 antikoerper, PLEKHH1L antikoerper, AI256725 antikoerper, E030001K05 antikoerper, mKIAA2028 antikoerper, pleckstrin homology, MyTH4 and FERM domain containing H2 antikoerper, pleckstrin homology domain containing, family H (with MyTH4 domain) member 2 antikoerper, Plekhh2 antikoerper, plekhh2 antikoerper, PLEKHH2 antikoerper
- Hintergrund
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Name/Gene ID: PLEKHH2
Synonyms: PLEKHH2, KIAA2028, PLEKHH1L - Gen-ID
- 130271
- NCBI Accession
- NP_742066
- UniProt
- Q8IVE3
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