NR2C1 Antikörper (N-Term)

Produktnummer ABIN6741570

Produktdetails anti-NR2C1 Antikörper

Target: NR2C1 (Nuclear Receptor Subfamily 2, Group C, Member 1 (NR2C1))

Bindungsspezifität: N-Term

Reaktivität: Human, Maus, Ratte, Hund, Pferd, Kaninchen, Schwein, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NR2C1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human NR2C1 / TR2-11

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Rabbit, Horse, Pig (100%) Bovine, Guinea pig (92%) Chicken (84%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human NR2C1 (Q15626, NP_001027458). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Rabbit, Horse, Pig (100%), Hamster, Bovine, Opossum, Guinea pig, Platypus, Lizard, Zebrafish (92%), Bat, Turkey, Zebra finch, Chicken, Xenopus (84%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu NR2C1

Target: NR2C1 (Nuclear Receptor Subfamily 2, Group C, Member 1 (NR2C1))

Andere Bezeichnung: NR2C1 (NR2C1 Produkte)

Synonyme: TR2 antikoerper, NR2C1 antikoerper, dor2 antikoerper, tr2-11 antikoerper, xDOR2 antikoerper, DKFZp468M0512 antikoerper, si:dkey-183c16.2 antikoerper, zgc:110672 antikoerper, 4831444H07Rik antikoerper, 80.3 antikoerper, Eenr antikoerper, Tr2-11 antikoerper, Tr2 antikoerper, Psg4 antikoerper, TR2-11 antikoerper, PSG9 antikoerper, nr2c1 antikoerper, nuclear receptor subfamily 2 group C member 1 antikoerper, nuclear receptor subfamily 2 group C member 1 S homeolog antikoerper, nuclear receptor subfamily 2, group C, member 1 antikoerper, pregnancy specific beta-1-glycoprotein 4 antikoerper, nuclear receptor subfamily 2 group C member 1 L homeolog antikoerper, NR2C1 antikoerper, nr2c1.S antikoerper, nr2c1 antikoerper, Nr2c1 antikoerper, PSG4 antikoerper, nr2c1.L antikoerper

Hintergrund: Name/Gene ID: NR2C1
Subfamily: NR2 Hepatocyte NF4-like
Family: NHR

Synonyms: NR2C1, Orphan nuclear receptor TR2, Testicular orphan receptor-2, TR2, TR2 nuclear hormone receptor, TR2-11, Testicular receptor 2

Gen-ID: 7181

NCBI Accession: NP_001027458

UniProt: P13056

Pathways: Nuclear Receptor Transcription Pathway, Retinoic Acid Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway