NR1I2 Antikörper (AA 108-157)

Produktnummer ABIN6741564

Produktdetails anti-NR1I2 Antikörper

Target: NR1I2 (Nuclear Receptor Subfamily 1, Group I, Member 2 (NR1I2))

Bindungsspezifität: AA 108-157

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NR1I2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human NR1I2 / PXR

Homologie: Percent identity by BLAST analysis: Human (100%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa108-157 of human NR1I2 (O75469, NP_003880). Percent identity by BLAST analysis: Human, Gorilla (100%), Gibbon, Monkey (85%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu NR1I2

Target: NR1I2 (Nuclear Receptor Subfamily 1, Group I, Member 2 (NR1I2))

Andere Bezeichnung: NR1I2 / PXR / PAR (NR1I2 Produkte)

Synonyme: BXR antikoerper, ONR1 antikoerper, PAR antikoerper, PAR1 antikoerper, PAR2 antikoerper, PARq antikoerper, PRR antikoerper, PXR antikoerper, SAR antikoerper, SXR antikoerper, PXR.1 antikoerper, PXR.2 antikoerper, PXR1 antikoerper, mPXR antikoerper, bxr antikoerper, nr1i2 antikoerper, onr1 antikoerper, xonr1 antikoerper, nuclear receptor subfamily 1 group I member 2 antikoerper, nuclear receptor subfamily 1, group I, member 2 antikoerper, nuclear receptor subfamily 1 group I member 2 S homeolog antikoerper, NR1I2 antikoerper, Nr1i2 antikoerper, nr1i2.S antikoerper, nr1i2 antikoerper

Hintergrund: Name/Gene ID: NR1I2
Subfamily: NR1 Thyroid hormone-like
Family: NHR

Synonyms: NR1I2, BXR, Orphan nuclear receptor PXR, Orphan nuclear receptor PAR1, PARq, PRR, PXR, Pregnane X receptor, ONR1, SAR, SXR

Gen-ID: 8856

NCBI Accession: NP_003880

UniProt: O75469

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway