Ziege anti-Affe IgM (Chain mu) Antikörper (DyLight 800) - Preadsorbed

Produktnummer ABIN6698970

Produktdetails

Target: IgM

Bindungsspezifität: Chain mu

Reaktivität: Affe

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: DyLight 800

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)

Produktmerkmale:

Synonyms: Goat Anti-Monkey IgM Antibody DyLight™ 800 Conjugation, Goat Anti-Monkey IgM DyLight™800 Conjugated Antibody

Background: Anti-Monkey IgM (mu chain) antibody generated in goat detects specifically monkey IgM. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. Anti-Monkey IgM antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.

Aufreinigung: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: Monkey IgM whole molecule

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:500 - 1:2,500

Application Note: Anti-Monkey IgM (mu chain) is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation. Specific conditions for reactivity and signal detection should be optimized by the end user.

FLISA Dilution: >1:20,000

ELISA Dilution: 1:10,000 - 1:50,000

Western Blot Dilution: 1:1,000 - 1:5,000

IF Microscopy Dilution: >1:5,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Antigendetails

Target: IgM

Abstract: IgM Produkte

Substanzklasse: Antibody