RAF1 Antikörper (pSer301)

Produktnummer ABIN6658142

Hersteller Produkt- Nr.: 600-401-e23

Der Kaninchen Polyklonal anti-RAF1 Antikörper (ABIN6658142) detektiert spezifisch RAF1 in WB. Dieser Antikörper reagiert spezifisch mit Proben aus Ratte.

Kurzübersicht für RAF1 Antikörper (pSer301) (ABIN6658142)

Target: RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RAF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-RAF1 Antikörper

Bindungsspezifität: pSer301

Hersteller Produkt- Nr.: 600-401-e23

Hersteller: Rockland

Verwendungszweck: Raf-1 phospho S301 Antibody

Kreuzreaktivität (Details): Reactivity is expected the following species based on 100 % sequence homology: bovine, canine, chicken, mouse, non-human primate and Xenopus.

Aufreinigung: Anti-Raf-1 pS301 antibodies are affinity purified from monospecific antiserum by immunoaffinity purification.

Immunogen: Anti-Raf-1 pS301 Antibody was produced in rabbit by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser301 conjugated to KLH.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optional[Neutralization_Dilution]: 1:1000

Kommentare:

Anti-Raf-1 pS301antibody is tested for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 74 kDa in size corresponding to the Raf-1 protein phosphorylated at Ser301 in the appropriate cell lysate or extract. Immunolabeling of the Raf-1 protein band is blocked by lambda-phosphatase treatment.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu RAF1

Target: RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))

Andere Bezeichnung: Raf-1

Hintergrund:

Synonyms: RAF proto-oncogene serine/threonine-protein kinase, Proto-oncogene c-RAF, cRaf

Background: Raf-1 pS301 antibody detects phosphorylated Raf-1. The Ras pathway is a critical signal transduction cascade involved in regulating cellular proliferation, differentiation, survival, and oncogenic transformation. Members of the Raf serine/threonine kinase family are key intermediates in this cascade, functioning to relay signals from activated Ras to the downstream protein kinases MEK and ERK. Previous studies have shown that phosphorylation is required for Raf-1 activation. Recent work has demonstrated that phosphorylation also regulates the downregulation of Raf with two sites participating: Ser301 and Ser642. Anti-Raf-1 pS301 Antibody is ideal for investigators involved in Cancer, Neuroscience, Signal Transduction research.

Gene Name: RAF1

Gen-ID: 24703

UniProt: P11345

Pathways: MAPK Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagie, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling, BCR Signaling