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Cyclin L1/L2 Antikörper

CCNL1/2 Reaktivität: Human IP, IHC, ELISA, WB Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN6656457
  • Target
    Cyclin L1/L2 (CCNL1/2)
    Reaktivität
    Human
    Wirt
    Kaninchen
    Klonalität
    Polyklonal
    Applikation
    Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
    Kreuzreaktivität
    Human, Maus, Ratte (Rattus)
    Aufreinigung
    The product was purified from monospecific antiserum by Protein A chromatography. This antibody will react with all forms of Cyclin L b (cyclin L1b and cyclin L2b).   Cross reactivity with Cyclin L beta proteins from other sources is unknown.
    Immunogen

    Immunogen: This Protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic construct consisting of full length Human Cyclin L1b protein.

    Immunogen Type: Recombinant Protein

    Isotyp
    IgG
  • Applikationshinweise

    Immunohistochemistry Dilution: 2 mg/mL - 20 μg/mL

    Application Note: This Protein A purified antibody has been tested for use in ELISA, western blot, immunohistochemistry, and immunoprecipitation.   Specific conditions for reactivity should be optimized by the end user.  Expect a band ~ 25 kDa to 35 kDa in size corresponding to Cyclin L1b and Cyclin L2b by western blotting in the appropriate cell lysate or extract.

    Immunoprecipitation Dilution: 1:100

    ELISA Dilution: 1:5,000 - 1:25,000

    Western Blot Dilution: 1:1,000 - 1:5,000

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    0.01 % (w/v) Sodium Azide

    Stabilizer: None

    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    RT,4 °C,-20 °C
    Informationen zur Lagerung
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
  • Loyer, Trembley, Katona, Kidd, Lahti: "Role of CDK/cyclin complexes in transcription and RNA splicing." in: Cellular signalling, Vol. 17, Issue 9, pp. 1033-51, (2005) (PubMed).

  • Target
    Cyclin L1/L2 (CCNL1/2)
    Andere Bezeichnung
    Cyclin L1/L2
    Hintergrund

    Synonyms: rabbit anti-Cyclin L1/L2 Antibody, rabbit anti-Cyclin L1 Antibody, rabbit anti-Cyclin L2 Antibody, Cyclin L beta, CCNL1, CCNL2, Paneth cell-enhanced expression protein

    Background: Cyclin L (also referred to as CCNL) is encoded by two highly related genes Cyclin L1 and Cyclin L2 (CCNL1 and CCNL2, respectively). Cyclin L has been shown to associate with the PITSLRE kinase and is involved in pre-mRNA processing. Both Cyclin L gene products can be alternatively spliced to produce two isoforms (known as 1 and 2 or alpha and beta). Cyclin L2 is ubiquitous, expressed at much higher levels than Cyclin L1, and thus is likely the major partner for the CDK11p110 protein kinase.

    Gene Name: CCNL1, CCNL2

    Gen-ID
    57018
    NCBI Accession
    NP_064703
    UniProt
    Q9UK58
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