POLR2A/RPB1 Antikörper
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- Target Alle POLR2A/RPB1 (POLR2A) Antikörper anzeigen
- POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))
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Reaktivität
- Human
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser POLR2A/RPB1 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), ELISA, Chromatin Immunoprecipitation (ChIP), Fluorescence Microscopy (FM)
- Aufreinigung
- Anti-Pol II Antibody was purified by Protein A chromatography. Cross-reactivity with Pol II from other sources has not been determined.
- Immunogen
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Immunogen: Anti-Pol II Antibody was produced in mice by repeated immunization with the YSPTSPS repeat in the B1 subunit of RNA polymerase II.
Immunogen Type: Peptide
- Isotyp
- IgG1
- Top Product
- Discover our top product POLR2A Primärantikörper
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- Applikationshinweise
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Application Note: Anti-Pol II Antibody is suitable for Chromatin Immunoprecipitation Sequencing, Chromatin Immunoprecipitation, ELISA, Immunofluorescence and Western Blots. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 225 kDa in the appropriate cell lysate or extract.
ChIP Dilution: 1 μg/ChIP
ELISA Dilution: 1:3,000
Western Blot Dilution: 1:1,000
IF Microscopy Dilution: 1:500
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Buffer
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Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
0.02 % (w/v) Sodium AzideStabilizer: None
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- RT,4 °C,-20 °C
- Informationen zur Lagerung
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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- Target
- POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))
- Andere Bezeichnung
- Pol II (POLR2A Produkte)
- Synonyme
- NCU02103.1 antikoerper, 19.m02399 antikoerper, POLR2A antikoerper, 220kDa antikoerper, Rpb1 antikoerper, Rpo2-1 antikoerper, POLR2 antikoerper, POLRA antikoerper, RPB1 antikoerper, RPBh1 antikoerper, RPO2 antikoerper, RPOL2 antikoerper, RpIILS antikoerper, hRPB220 antikoerper, hsRPB1 antikoerper, DNA-directed RNA polymerase II largest subunit antikoerper, DNA-directed RNA polymerase II subunit RPB1 antikoerper, RNA polymerase II large subunit Rpb1 antikoerper, RNA polymerase II subunit A antikoerper, DNA-directed RNA polymerase II largest subunit, putative antikoerper, polymerase (RNA) II (DNA directed) polypeptide A, 220kDa antikoerper, polymerase (RNA) II (DNA directed) polypeptide A antikoerper, NCU02103 antikoerper, BBOV_I003360 antikoerper, EDI_169920 antikoerper, CMU_000800 antikoerper, SJAG_01713 antikoerper, MGYG_01527 antikoerper, POLR2A antikoerper, TP03_0185 antikoerper, EBI_22514 antikoerper, CGB_E5020W antikoerper, polr2a antikoerper, Polr2a antikoerper
- Hintergrund
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Synonyms: DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, DNA directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1, POLR2, RNAPII
Background: RNA polymerase II (pol II) is a key enzyme in the regulation and control of gene transcription. It is able to unwind the DNA double helix, synthesize RNA, and proofread the result. Pol II is a complex enzyme, consisting of 12 subunits, of which the B1 subunit is the largest. Together with the second largest subunit, B1 forms the catalytic core of the RNA polymerase II transcription machinery. Anti-Pol II Antibody is ideal for research in Gene Expression, Transcription and Genetics.
Gene Name: POLR2A
- Gen-ID
- 5430
- NCBI Accession
- NP_000928
- UniProt
- P24928
- Pathways
- Regulatorische RNA Pathways
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