P27 (AA 140-220) Antikörper

Produktnummer ABIN6281250

Produktdetails

Target: P27

Bindungsspezifität: AA 140-220

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), ELISA

Verwendungszweck: p27 is a protein encoded by the CDKN1B gene which is approximately 22 kDa. p27 is localised to the nucleus and cytoplasm. It is involved in CDK-mediated phosphorylation and removal of Cdc6, RET signalling, GAB1 signalosome and cell cycle role of SCF complex in cell cycle regulation. It is a cyclin-dependent kinase inhibitor that binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, which are important in controlling the cell cycle progression at G1. p27 is expressed in all tissues with the highest levels in skeletal muscles. Mutations in the CDKN1B gene may result in multiple endocrine neoplasia. STJ94866 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of p27 protein.

Spezifität: P27 Polyclonal Antibody detects endogenous levels of p27 protein.

Produktmerkmale: Rabbit polyclonal to p27.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human p27 around the non-phosphorylation site of T198.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:2000
ELISA 1:5000

Kommentare:

Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C, and avoid repeat freeze-thaw cycles.

Antigendetails

Target: P27

Synonyme: Kip1 antikoerper, p27 antikoerper, cyclin dependent kinase inhibitor 1B antikoerper, CDKN1B antikoerper

Substanzklasse: Viral Protein

Molekulargewicht: 27 kDa

Gen-ID: 1027

UniProt: P46527