PRKAG1/2/3 Antikörper

Produktnummer ABIN6259312

Produktdetails anti-PRKAG1/2/3 Antikörper

Target: PRKAG1/2/3 (Protein Kinase, AMP-Activated, gamma 1/2/3 Non-Catalytic Subunit (PRKAG1/2/3))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PRKAG1/2/3 Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Spezifität: PRKAG1/2/3 Antibody detects endogenous levels of total PRKAG1/2/3

Kreuzreaktivität: Human, Maus, Ratte (Rattus)

Aufreinigung: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500~1:1000, IF/ICC 1:100-1:500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C.Stable for 12 months from date of receipt

Haltbarkeit: 12 months

Details zu PRKAG1/2/3

Target: PRKAG1/2/3 (Protein Kinase, AMP-Activated, gamma 1/2/3 Non-Catalytic Subunit (PRKAG1/2/3))

Andere Bezeichnung: PRKAG1/2/3 (PRKAG1/2/3 Produkte)

Hintergrund:

Description: AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton, probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive.

Gene: PRKAG1

Molekulargewicht: 38 kDa

Gen-ID: 5571

UniProt: P54619, Q9UGJ0, Q9UGI9