GNAI3 Antikörper (N-Term)

Produktnummer ABIN6258933

Produktdetails anti-GNAI3 Antikörper

Target: GNAI3 (Guanine Nucleotide Binding Protein (G Protein), alpha Inhibiting Activity Polypeptide 3 (GNAI3))

Bindungsspezifität: N-Term

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GNAI3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Spezifität: GNAI3 Antibody detects endogenous levels of total GNAI3.

Homologie: Pig,Zebrafish,Bovine,Sheep,Rabbit,Dog,Xenopus

Aufreinigung: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human GNAI3, corresponding to a region within N-terminal amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C. Stable for 12 months from date of receipt.

Haltbarkeit: 12 months

Referenzen für anti-GNAI3 Antikörper (ABIN6258933)

Costas-Insua, Moreno, Maroto, Ruiz-Calvo, Bajo-Grañeras, Martín-Gutiérrez, Diez-Alarcia, Vilaró, Cortés, García-Font, Martín, Espina, Botta, Ginés, McCormick, Sánchez-Prieto, Galve-Roperh, Mengod et al.: "Identification of BiP as a CB1 Receptor-Interacting Protein That Fine-Tunes Cannabinoid Signaling in the Mouse Brain. ..." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 41, Issue 38, pp. 7924-7941, (2021) (PubMed).

Details zu GNAI3

Target: GNAI3 (Guanine Nucleotide Binding Protein (G Protein), alpha Inhibiting Activity Polypeptide 3 (GNAI3))

Andere Bezeichnung: GNAI3 (GNAI3 Produkte)

Synonyme: AI158965 antikoerper, AW537698 antikoerper, Galphai3 antikoerper, Gnai-3 antikoerper, 87U6 antikoerper, ARCND1 antikoerper, GIP3A antikoerper, G protein subunit alpha i3 antikoerper, guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 3 antikoerper, guanine nucleotide binding protein (G protein), alpha inhibiting 3 antikoerper, G protein subunit alpha i3 S homeolog antikoerper, GNAI3 antikoerper, gnai3 antikoerper, Gnai3 antikoerper, gnai3.S antikoerper

Hintergrund:

Description: Heterotrimeric guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins (PubMed:8774883, PubMed:18434541, PubMed:19478087). Signaling is mediated via effector proteins, such as adenylate cyclase. Inhibits adenylate cyclase activity, leading to decreased intracellular cAMP levels (PubMed:19478087). Stimulates the activity of receptor-regulated K+ channels (PubMed:2535845). The active GTP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division (PubMed:17635935).

Gene: GNAI3

Molekulargewicht: 41 kDa

Gen-ID: 2773

UniProt: P08754

Pathways: cAMP Metabolic Process, G-protein mediated Events