AAK1 Antikörper (Internal Region)

Produktnummer ABIN6257901

Produktdetails anti-AAK1 Antikörper

Target: AAK1 (AP2 associated kinase 1 (AAK1))

Bindungsspezifität: Internal Region

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser AAK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Spezifität: AAK1 Antibody detects endogenous levels of total AAK1.

Homologie: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken

Aufreinigung: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human AAK1, corresponding to a region within the internal amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C. Stable for 12 months from date of receipt.

Haltbarkeit: 12 months

Details zu AAK1

Target: AAK1 (AP2 associated kinase 1 (AAK1))

Andere Bezeichnung: AAK1 (AAK1 Produkte)

Synonyme: 5530400K14Rik antikoerper, 9630042K20 antikoerper, AU067724 antikoerper, AU067726 antikoerper, BC028270 antikoerper, C79663 antikoerper, D6Ertd245e antikoerper, R75501 antikoerper, mKIAA1048 antikoerper, RGD1563580 antikoerper, aak1 antikoerper, si:dkey-261l11.5 antikoerper, AP2 associated kinase 1 antikoerper, uncharacterized protein FLJ45252 antikoerper, AP2 associated kinase 1b antikoerper, AAK1 antikoerper, Aak1 antikoerper, LOC100512341 antikoerper, aak1b antikoerper

Hintergrund:

Description: Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis. Isoform 1 and isoform 2 display similar levels of kinase activity towards AP2M1. Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes. Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes. Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity.

Gene: AAK1

Molekulargewicht: 94 kDa

Gen-ID: 22848

UniProt: Q2M2I8