TYR Antikörper
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- Target Alle TYR Antikörper anzeigen
- TYR (Tyrosinase (TYR))
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Reaktivität
- Human
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser TYR Antikörper ist unkonjugiert
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Applikation
- Immunofluorescence (IF), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Aufreinigung
- Protein G affinity chromatography
- Immunogen
- Recombinant human protein was used as the immunogen for the Tyrosinase antibody.
- Klon
- OCA1-812
- Isotyp
- IgG2a kappa
- Top Product
- Discover our top product TYR Primärantikörper
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- Applikationshinweise
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ELISA (order Ab without BSA)
Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)
Optimal dilution of the Tyrosinase antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, or 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 0.2 mg/mL
- Buffer
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- Target
- TYR (Tyrosinase (TYR))
- Andere Bezeichnung
- Tyrosinase (TYR Produkte)
- Synonyme
- sandy antikoerper, zgc:109705 antikoerper, TYR antikoerper, TYRO antikoerper, tyr antikoerper, LOC100136546 antikoerper, CMM8 antikoerper, OCA1A antikoerper, OCAIA antikoerper, SHEP3 antikoerper, albino antikoerper, c antikoerper, skc35 antikoerper, C antikoerper, tyrosinase antikoerper, TYRosinase antikoerper, tyrosinase S homeolog antikoerper, tyr antikoerper, tyr-5 antikoerper, tyr.S antikoerper, TYR antikoerper, Celal_3121 antikoerper, Halhy_4121 antikoerper, Mesop_2736 antikoerper, LOC100136546 antikoerper, Tyr antikoerper
- Hintergrund
- Recognizes a cluster of proteins between 70-80 kDa, identified as tyrosinase. Occasionally a minor band at 55 kDa is also detected. This mAb shows no cross-reaction with MAGE-1 and tyrosinase-related protein 1, TRP-1/gp75. Tyrosinase is a copper-containing metalloglycoprotein that catalyzes several steps in the melanin pigment biosynthetic pathway, the hydroxylation of tyrosine to L-3,4-dihydroxy-phenylalanine (dopa), and the subsequent oxidation of dopa to dopaquinone. Mutations of the tyrosinase gene occur in various forms of albinism. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Staining of melanomas with this mAb shows tyrosinase in melanotic as well as amelanotic variants. This mAb is a useful marker for melanocytes and melanomas.
- Gen-ID
- 7299
- UniProt
- P14679
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