TFF1 Antikörper (AA 57-84)

Produktnummer ABIN6251840

Produktdetails anti-TFF1 Antikörper

Target: TFF1 (Trefoil Factor 1 (TFF1))

Bindungsspezifität: AA 57-84

Reaktivität: Human, Cynomolgus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TFF1 Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Aufreinigung: Protein G affinity chromatography

Immunogen: Amino acids CFDDTVRGVPWCFYPNTIDVPPEEECEF (aa57-84) from the human protein were used as the immunogen for the Estrogen Inducible Protein pS2 antibody.

Klon: SPM313

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 0.5-1 μg/mL

Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Prediluted format: incubate for 30 min at RT (2)

Optimal dilution of the Estrogen Inducible Protein pS2 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Details zu TFF1

Target: TFF1 (Trefoil Factor 1 (TFF1))

Andere Bezeichnung: Estrogen Inducible Protein pS2 (TFF1 Produkte)

Synonyme: BCEI antikoerper, D21S21 antikoerper, HP1.A antikoerper, HPS2 antikoerper, pNR-2 antikoerper, pS2 antikoerper, Bcei antikoerper, PS2 antikoerper, bcei antikoerper, d21s21 antikoerper, hp1.a antikoerper, hps2 antikoerper, p1 antikoerper, p1-a antikoerper, pnr-2 antikoerper, ps2 antikoerper, tff1 antikoerper, xP1 antikoerper, xp1-L antikoerper, xp1 antikoerper, TFF1 antikoerper, Ps2 antikoerper, trefoil factor 1 antikoerper, trefoil factor 1 S homeolog antikoerper, trefoil factor 1, gene 2 S homeolog antikoerper, TFF1 antikoerper, Tff1 antikoerper, tff1.S antikoerper, tff1.2.S antikoerper, LOC100358238 antikoerper

Hintergrund: It recognizes a polypeptide of 6.5  kDa, identified as pS2 estrogen-regulated protein. Its epitope is localized between aa57-84 of human pS2 protein. pS2 is a trefoil peptide. Trefoil peptides are protease resistant molecules secreted throughout the gut that play a role in mucosal healing. These peptides contain three intra-chain disulfide bonds, forming the trefoil motif, or P-domain. pS2 is known to form dimers and this dimerization is thought to play a role in its protective and healing properties. About 60 % of breast carcinomas are positive for pS2. Staining is cytoplasmic, often with localization to the Golgi apparatus. pS2 is shown to be localized in normal stomach mucosa, gastric fluid, goblet cells in the colon and small intestine, and in ulcerations of the gastrointestinal tract. Several studies have shown that pS2 is primarily expressed in estrogen receptor-positive breast tumors and it may define a subset of estrogen-dependent tumors that displays an increased likelihood of response to endocrine therapy.

Gen-ID: 7031

UniProt: P04155

Pathways: EGFR Signaling Pathway