LEO1 Antikörper (C-Term)

Produktnummer ABIN6242423

Produktdetails anti-LEO1 Antikörper

Target: LEO1 (Leo1, Paf1/RNA Polymerase II Complex Component, Homolog (S. Cerevisiae) (LEO1))

Bindungsspezifität: AA 616-650, C-Term

Reaktivität: Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LEO1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Aufreinigung: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This Mouse Leo1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 616-650 amino acids from the C-terminal region of Mouse Leo1.

Klon: RB50947

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: WB: 1:1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Haltbarkeit: 6 months

Details zu LEO1

Target: LEO1 (Leo1, Paf1/RNA Polymerase II Complex Component, Homolog (S. Cerevisiae) (LEO1))

Andere Bezeichnung: Leo1 (LEO1 Produkte)

Synonyme: LEO1 antikoerper, RDL antikoerper, Gm185 antikoerper, zgc:77374 antikoerper, LEO1 homolog, Paf1/RNA polymerase II complex component antikoerper, Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) antikoerper, RNA polymerase-associated protein LEO1 antikoerper, LEO1 homolog, Paf1/RNA polymerase II complex component S homeolog antikoerper, Leo1, Paf1/RNA polymerase II complex component antikoerper, LEO1 antikoerper, leo1 antikoerper, LOC100466503 antikoerper, leo1.S antikoerper, Leo1 antikoerper, LOC415420 antikoerper, LOC478310 antikoerper

Hintergrund: Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non- phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys- 4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1), UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. Involved in polyadenylation of mRNA precursors. Connects PAF1C to Wnt signaling (By similarity).

Molekulargewicht: 75597

UniProt: Q5XJE5

Pathways: Stem Cell Maintenance