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Histone 3 Antikörper

H3 Reaktivität: Tomato, Arabidopsis thaliana, Mais (Zea mays), Chlamydomonas reinhardtii, Moss WB, ICC Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN619545
  • Target Alle Histone 3 (H3) Antikörper anzeigen
    Histone 3 (H3)
    Reaktivität
    • 1377
    • 940
    • 861
    • 59
    • 42
    • 41
    • 41
    • 40
    • 39
    • 35
    • 35
    • 30
    • 26
    • 22
    • 19
    • 6
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Tomato, Arabidopsis thaliana, Mais (Zea mays), Chlamydomonas reinhardtii, Moss
    Wirt
    • 1183
    • 246
    • 12
    • 4
    Kaninchen
    Klonalität
    • 1108
    • 336
    • 1
    Polyklonal
    Konjugat
    • 797
    • 81
    • 65
    • 64
    • 63
    • 63
    • 63
    • 62
    • 34
    • 33
    • 22
    • 17
    • 17
    • 17
    • 17
    • 17
    • 12
    • 1
    Dieser Histone 3 Antikörper ist unkonjugiert
    Applikation
    • 1034
    • 402
    • 373
    • 311
    • 240
    • 188
    • 175
    • 171
    • 163
    • 151
    • 136
    • 127
    • 117
    • 48
    • 38
    • 34
    • 32
    • 20
    • 16
    • 14
    • 7
    • 7
    • 6
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunocytochemistry (ICC)
    Homologie
    dicots including: Vitis vinifera, monocots: Hordeum vulgare, Oryza sativa, Zea mays, trees: Pinus pinaster, algae: Volvox sp.
    Produktmerkmale
    Expected / apparent Molecular Weight of the Antigene: 15 / 17 kDa
    Aufreinigung
    serum
    Immunogen
    KLH-conjugated synthetic peptide derived from known H3 sequences, inluding Arabidopsis thaliana H3.3 P59169 (At4g40030,At4g40040,At4g10980), H3.2 P59226(At4g09200,At4g27360,At4g10390,At4g10400,At4g65360), H3-like 2 Q9FXI7 (At4g19890)
    Top Product
    Discover our top product H3 Primärantikörper
  • Applikationshinweise
    Recommended Dilution: 1 : 5000 with standard ECL (WB), 1 : 100 - 1 : 500 (ICC)
    Kommentare

    Protocol for isolation of cytosolic and nuclear fractions can be found here.Specific fluorescence in ICC has been observed for interphase nuclei as well as around centromer region (where Ser10 of histone H3 is phosphorylated) in mitotic chromosomes.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
  • Sikorskaite, Rajamäki, Baniulis, Stanys, Valkonen: "Protocol: Optimised methodology for isolation of nuclei from leaves of species in the Solanaceae and Rosaceae families." in: Plant methods, Vol. 9, Issue 1, pp. 31, (2013) (PubMed).

    van der Linde, Hemetsberger, Kastner, Kaschani, van der Hoorn, Kumlehn, Doehlemann: "A maize cystatin suppresses host immunity by inhibiting apoplastic cysteine proteases." in: The Plant cell, Vol. 24, Issue 3, pp. 1285-300, (2012) (PubMed).

  • Target
    Histone 3 (H3)
    Andere Bezeichnung
    H3 (H3 Produkte)
    Synonyme
    H-3 antikoerper, histocompatibility 3 antikoerper, H3 antikoerper
    Hintergrund
    AGI Code: At4g40030
    Histone 3 (H3) located in nuclei, incorporated into chromatin. Present in nucleosome together with H2A, H2B and H4.
    Molekulargewicht
    expected: 15 kDa, apparent: 17 kDa
    UniProt
    P59169, P59226, Q9FXI7
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