TYR Antikörper
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- Target Alle TYR Antikörper anzeigen
- TYR (Tyrosinase (TYR))
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Reaktivität
- Human
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser TYR Antikörper ist unkonjugiert
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Applikation
- Immunofluorescence (IF), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Coating (Coat)
- Verwendungszweck
- Mouse Monoclonal anti-Tyrosinase (OCA1/812), Purified, BSA-free
- Produktmerkmale
- This antibody recognizes a cluster of proteins between 70-80 kDa, identified as tyrosinase. Occasionally a minor band at 55 kDa is also detected. This MAb shows no cross-reaction with MAGE-1 and tyrosinase-related protein 1, TRP-1/gp75. Tyrosinase is a copper-containing metalloglycoprotein that catalyzes several steps in the melanin pigment biosynthetic pathway, the hydroxylation of tyrosine to L-3,4-dihydroxy-phenylalanine (dopa), and the subsequent oxidation of dopa to dopaquinone. Mutations of the tyrosinase gene occur in various forms of albinism. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Staining of melanomas with this MAb shows tyrosinase in melanotic as well as amelanotic variants. This MAb is a useful marker for melanocytes and melanomas. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Aufreinigung
- Purified
- Immunogen
- Recombinant human TYR protein
- Klon
- OCA1-812
- Isotyp
- IgG2a kappa
- Top Product
- Discover our top product TYR Primärantikörper
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- Applikationshinweise
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Immunohistology formalin-fixed 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, or 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 1-2 μg/mL
- ELISA For coating, order Ab without BSA
- Optimal dilution for a specific application should be determined by user
- Kommentare
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SK-MEL-13, SK-MEL-19, SK-MEL-30, SK-MEL-37 cells or Melanoma.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 1 mg/mL
- Buffer
- PBS (no BSA, no azide)
- Konservierungsmittel
- Azide free
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- Target
- TYR (Tyrosinase (TYR))
- Andere Bezeichnung
- Tyrosinase (TYR Produkte)
- Synonyme
- sandy antikoerper, zgc:109705 antikoerper, TYR antikoerper, TYRO antikoerper, tyr antikoerper, LOC100136546 antikoerper, CMM8 antikoerper, OCA1A antikoerper, OCAIA antikoerper, SHEP3 antikoerper, albino antikoerper, c antikoerper, skc35 antikoerper, C antikoerper, tyrosinase antikoerper, TYRosinase antikoerper, tyrosinase S homeolog antikoerper, tyr antikoerper, tyr-5 antikoerper, tyr.S antikoerper, TYR antikoerper, Celal_3121 antikoerper, Halhy_4121 antikoerper, Mesop_2736 antikoerper, LOC100136546 antikoerper, Tyr antikoerper
- Hintergrund
- ATN, CMM8, LB24-AB, Monophenol monooxygenase, OCA1, OCA1A, Oculocutaneous albinism IA, SHEP3, SK29-AB, Tumor rejection antigen AB, TYR
- Molekulargewicht
- 70-80 kDa
- Gen-ID
- 7299, 503555
- UniProt
- P14679
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