MITF Antikörper (CF®405S)
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- Target Alle MITF Antikörper anzeigen
- MITF (Microphthalmia-Associated Transcription Factor (MITF))
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Reaktivität
- Human
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser MITF Antikörper ist konjugiert mit CF®405S
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Applikation
- Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
- Verwendungszweck
- Mouse Monoclonal anti-MiTF (Microphthalmia Transcription Factor) (D5 + MITF/915), CF405S Conjugate
- Produktmerkmale
- MITF (microphthalmia transcription factor) is a basic helix-loop-helix-leucine-zipper (bHLH-Zip) transcription factor that regulates the development and survival of melanocytes and retinal pigment epithelium, and also is involved in transcription of pigmentation enzyme genes such as tyrosinase TRP1 and TRP2. MITF has been shown to be phosphorylated by MAP kinase in response to c-kit activation, resulting in upregulation of MITF transcriptional activity. Mutations of the MITF gene are associated with the autosomal dominant hereditary deafness and pigmentation condition, Waardenburg Syndrome type 2A. Multiple isoforms of MITF exist, including MITF-A, MITF-B, MITF-C, MITF-H, and MITF-M, which differ in the amino-terminal domain and in their expression patterns. The MITF-M isoform is restricted to the melanocyte cell lineage. Anti-MITF, D5, recognizes a nuclear protein, which is expressed in the majority of primary and metastatic epithelioid malignant melanomas as well as in normal melanocytes, benign nevi and dysplastic nevi. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogen
- NH2 terminus fragment of human Mi protein (D5), Recombinant human MiTF protein (MITF/915)
- Klon
- D5 MITF-915
- Isotyp
- IgG
- Top Product
- Discover our top product MITF Primärantikörper
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- Applikationshinweise
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Does not react with mouse or rat, others not tested
- Immunohistology (formalin)
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
- Kommentare
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Jurkat, A-431, HeLa or 501 Mel human melanoma cells or Melanoma
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Protect from light
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- Target
- MITF (Microphthalmia-Associated Transcription Factor (MITF))
- Andere Bezeichnung
- MiTF (MITF Produkte)
- Synonyme
- MITF antikoerper, ws2a antikoerper, mitfa antikoerper, MITF-A antikoerper, LOC100313655 antikoerper, mitfb antikoerper, CMM8 antikoerper, MI antikoerper, WS2 antikoerper, WS2A antikoerper, bHLHe32 antikoerper, BCC2 antikoerper, Bhlhe32 antikoerper, Gsfbcc2 antikoerper, Vitiligo antikoerper, Wh antikoerper, bw antikoerper, mi antikoerper, vit antikoerper, MITF-H antikoerper, MITF-M antikoerper, CMI9 antikoerper, melanogenesis associated transcription factor antikoerper, microphthalmia-associated transcription factor antikoerper, microphthalmia-associated transcription factor S homeolog antikoerper, Microphthalmia antikoerper, MITF antikoerper, mitf antikoerper, LOC100313655 antikoerper, mitf.S antikoerper, LOC100534525 antikoerper, MICROP antikoerper, Mitf antikoerper
- Hintergrund
- BHLHE32, Class E basic helix-loop-helix protein 32 (bHLHe32), CMM8, Mi, Microphthalmia-associated transcription factor, MITF, WS2, WS2A
- Molekulargewicht
- 52-56 kDa (doublet)
- Gen-ID
- 4286
- UniProt
- O75030
- Pathways
- Chromatin Binding
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