Rekombinanter CNN1 Antikörper (Biotin)

Produktnummer ABIN6178356

Produktdetails anti-CNN1 Antikörper (Biotin)

Target: CNN1 (Calponin 1 (CNN1))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser CNN1 Antikörper ist konjugiert mit Biotin

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)

Verwendungszweck: Recombinant Rabbit Monoclonal anti-Calponin-1 (CNN1/1408R), Biotin Conjugate

Produktmerkmale: Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist, a 34 kDa form and a 29 kDa form. Expression of the 29 kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. In Western blotting, this MAb reacts with only the 34 kDa form of calponin in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human full-length CNN1 protein

Klon: CNN1-1408R

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology (formalin) 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA  pH 7.5-8.5 for 10-20 min followed by cooling at RT for 20 min
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

Myoepithelial cells in breast ducts

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Details zu CNN1

Target: CNN1 (Calponin 1 (CNN1))

Andere Bezeichnung: Calponin-1 (CNN1 Produkte)

Synonyme: CN antikoerper, CnnI antikoerper, SMCC antikoerper, Sm-Calp antikoerper, XclpH3 antikoerper, clpH3 antikoerper, cnn2 antikoerper, calponin 1 antikoerper, calponin 1, basic, smooth muscle L homeolog antikoerper, cnn1 antikoerper, Cnn1 antikoerper, CNN1 antikoerper, cnn1.L antikoerper

Hintergrund: Calponin 1 basic smooth muscle, Calponin H1 smooth muscle, Calponin-1, CNN1, Cnn1, Sm Calp, SMCC

Molekulargewicht: 34 kDa

Gen-ID: 1264, 465929

UniProt: P51911