TYMS Antikörper

Produktnummer ABIN6171333

Produktdetails anti-TYMS Antikörper

Target: TYMS (Thymidylate Synthetase (TYMS))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TYMS Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Immunostaining (ISt), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Verwendungszweck: Mouse Monoclonal anti-Thymidylate Synthase (TS106), Purified, BSA-free

Produktmerkmale: This antibody recognizes a protein of 36 kDa, identified as Thymidylate Synthase (TS) (EC 2.1.1.45). TS converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is also a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein is associated with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Aufreinigung: Purified

Immunogen: Recombinant human thymidylate synthase

Klon: TS106

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology formalin-fixed 1-2 μg/mL

• Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

5-FU-resistant colon carcinoma cell lines (NCI H630R10, NCI H630R1), 5-FU-resistant breast cancer cell lines, MCF-Ad5 and MCF-Ad10. Testicular carcinomas.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS (no BSA, no azide)

Konservierungsmittel: Azide free

Details zu TYMS

Target: TYMS (Thymidylate Synthetase (TYMS))

Andere Bezeichnung: Thymidylate Synthase (TYMS Produkte)

Synonyme: FN1 antikoerper, ECK2823 antikoerper, JW2795 antikoerper, ts antikoerper, Ts antikoerper, HST422 antikoerper, TMS antikoerper, TS antikoerper, thymidylate synthetase antikoerper, contains intron in T4 phage antikoerper, ThyE antikoerper, thymidylate synthase antikoerper, thymidylate synthetase L homeolog antikoerper, TYMS antikoerper, thyA antikoerper, td antikoerper, thyE antikoerper, DDA3937_RS04960 antikoerper, tyms.L antikoerper, XBJ1_RS15770 antikoerper, EAMY_RS20680 antikoerper, Tyms antikoerper

Substanzklasse: Viral Protein

Hintergrund: DTMP synthase, TMS, TS, TSase, TYMS protein, Tyms thymidylate synthetase

Molekulargewicht: 36 kDa

Gen-ID: 7298, 369762

UniProt: P04818

Pathways: Mitotic G1-G1/S Phases