Myogenin Antikörper (AA 30-224)
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- Target Alle Myogenin (MYOG) Antikörper anzeigen
- Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))
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Bindungsspezifität
- AA 30-224
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Reaktivität
- Human, Maus, Ratte, Schwein, Cat
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser Myogenin Antikörper ist unkonjugiert
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Applikation
- Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
- Verwendungszweck
- Mouse Monoclonal anti-Myogenin (MGN185 + F5D), Purified, with BSA
- Produktmerkmale
- Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif. MyoD family members share about 80 % amino acid homology in their bHLH motifs. Anti-myogenin labels the nuclei of myoblasts in developing muscle tissue, and is expressed in tumor cell nuclei of rhabdomyosarcoma and some leiomyosarcomas. Positive nuclear staining may occur in Wilms? tumor. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Aufreinigung
- Purified
- Immunogen
- Human myogenin recombinant protein (MGN185), Rat myogenin recombinant fragment containing amino acid 30-224 (F5D)
- Klon
- MGN185 F5D
- Isotyp
- IgG
- Top Product
- Discover our top product MYOG Primärantikörper
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- Applikationshinweise
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Immunohistology (formalin)
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
- Kommentare
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Rh-30 cells. Skeletal muscle or rhabdomyosarcoma.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 200 μg/mL
- Buffer
- PBS/0.05 % BSA/0.05 % azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- Target
- Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))
- Andere Bezeichnung
- Myogenin (MYOG Produkte)
- Synonyme
- cb553 antikoerper, zgc:100763 antikoerper, LOC446037 antikoerper, myf4 antikoerper, Xmyog antikoerper, myogenin antikoerper, LOC100136471 antikoerper, LOC100224062 antikoerper, LOC100303673 antikoerper, MYF4 antikoerper, bHLHc3 antikoerper, myo antikoerper, myf-4 antikoerper, myogenin antikoerper, myogenin (myogenic factor 4) antikoerper, MYOG antikoerper, myog antikoerper, LOC100303673 antikoerper, Myog antikoerper
- Hintergrund
- BHLHc3, cb553, Class C basic helix-loop-helix protein 3, Myf-4, MYF4, MYOG, Myogenic factor 4
- Molekulargewicht
- 34 kDa
- Gen-ID
- 4656, 2830
- UniProt
- P15173
- Pathways
- Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
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