TNC Antikörper (CF®594)

Produktnummer ABIN6167842

Produktdetails anti-TNC Antikörper (CF®594)

Target: TNC (Tenascin C (TNC))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TNC Antikörper ist konjugiert mit CF®594

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Verwendungszweck: Mouse Monoclonal anti-Tenascin (T2H5), CF594 Conjugate

Produktmerkmale: In Western blotting, this antibody reacts with two bands of ~MW of 210 kDa and 300 kDa, identified as two isoforms of Tenascin C. Specificity of this MAb is validated by sequential immunoprecipitation with a PAb against Tenascin C. Tenascin C is a multifunctional, disulfide-linkedhexameric extracellular matrix glycoprotein expressed in association with mesenchymal epithelial interactions during development and in the neo-vasculature and stroma of undifferentiated tumors. In adults, it is restricted to certain epithelial-stromal interfaces and increases markedly in hyper-proliferative diseases and in stroma of many neoplasms, including gliomas, breast, squamous and lung carcinomas. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Human breast carcinoma

Klon: T2H5

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Does not react with rat, others not known

• Immunohistology formalin-fixed 2-4 μg/mL
• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

Extracellular matrix in tonsil and blood vessels. Stroma of many tumors such as breast, squamous cell, and lung carcinomas. Staining of normal fibroblasts serves as internal positive control

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu TNC

Target: TNC (Tenascin C (TNC))

Andere Bezeichnung: Tenascin (TNC Produkte)

Synonyme: tn antikoerper, 150-225 antikoerper, GMEM antikoerper, GP antikoerper, HXB antikoerper, JI antikoerper, TN antikoerper, TN-C antikoerper, AI528729 antikoerper, C130033P17Rik antikoerper, Hxb antikoerper, Ten antikoerper, cytotactin antikoerper, tenascin-C antikoerper, P230 antikoerper, tenc antikoerper, wu:fk04d02 antikoerper, tenascin C L homeolog antikoerper, tenascin C antikoerper, tnc.L antikoerper, TNC antikoerper, MCYG_00667 antikoerper, tnc antikoerper, Tnc antikoerper

Hintergrund: Cytotactin, Glioma-associated-extracellular matrix antigen, GMEM, GP 150-225, Hexabrachion (HXB), JI, Myotendinous antigen, Neuronectin, TNC

Molekulargewicht: 210 kDa and 300 kDa

Gen-ID: 3371, 143250

UniProt: P24821

Pathways: Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development