MVP Antikörper

Produktnummer ABIN6165384

Produktdetails anti-MVP Antikörper

Target: MVP (Major Vault Protein (MVP))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MVP Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Verwendungszweck: Mouse Monoclonal anti-Major Vault Protein (MVP) (1032), Purified, BSA-free

Produktmerkmale: This antibody recognizes a protein of 104 kDa-110 kDa, characterized as major vault protein (MVP). Vaults are large ribonucleoprotein particles (RNPs) present in all eukaryotic cells. They have a complex morphology, including several small molecules of RNA, but a single protein species. The MVP accounts for >70 % of their mass. Their shape is reminiscent of the nucleopore central plug. Treatment of cells with estradiol increases the amount of MVP in nuclear extract. The hormone-dependent interaction of vaults with ER is prevented in vitro by sodium molybdate. Antibodies to estrogen, progesterone and glucocorticoid receptors are able to co-immunoprecipitate the MVP. MVP is overexpressed in many neoplastic tissues and cell lines. Expression of MVP predicts a poor response to chemotherapy. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Aufreinigung: Purified

Immunogen: Proteins precipitated from human breast cancer MCF-7 cells

Klon: 1032

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

MCF-7/HeLa, breast tumors

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS (no BSA, no azide)

Konservierungsmittel: Azide free

Details zu MVP

Target: MVP (Major Vault Protein (MVP))

Abstract: MVP Produkte

Synonyme: LRP antikoerper, VAULT1 antikoerper, 2310009M24Rik antikoerper, cb771 antikoerper, wu:fb52b05 antikoerper, wu:fc02g01 antikoerper, mvp antikoerper, MGC145641 antikoerper, Tb05.45E22.810 antikoerper, major vault protein antikoerper, major vault protein L homeolog antikoerper, putative major vault protein antikoerper, MVP antikoerper, Mvp antikoerper, mvp antikoerper, mvp.L antikoerper, Tc00.1047053510353.10 antikoerper, Tb927.5.4460 antikoerper, Tb10.70.0520 antikoerper, Tb10.70.5840 antikoerper, LMJF_05_0060 antikoerper

Hintergrund: Lung resistance-related protein (LRP), Major Vault Protein, MVP, VAULT1

Molekulargewicht: 104-110 kDa

Gen-ID: 9961, 632177

UniProt: Q14764