Desmoglein 3 Antikörper (Biotin)

Produktnummer ABIN6162375

Produktdetails anti-Desmoglein 3 Antikörper (Biotin)

Target: Desmoglein 3 (DSG3)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Desmoglein 3 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Verwendungszweck: Mouse Monoclonal anti-Desmoglein-3 (DSG3/1535), Biotin Conjugate

Produktmerkmale: This antibody recognizes a protein of 130 kDa, identified as Desmoglein-3 (DSG3). This MAb is highly specific to Desmoglein-3 and does not cross-react with other members of the Desmoglein-family. DSG3 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Research has shown that DSG3 has a very high sensitivity (>80 % ) and specificity (100 %) in recognizing squamous cell carcinoma (SqCC). Therefore, DSG3 is considered a very important marker for lung SqCC and can be a useful ancillary marker to separate SqCC from other subtypes of lung cancer. Moreover, studies have shown that DSG3 expression in lung SqCC may indicate a poor prognosis. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human DSG3 extracellular protein fragment (exact sequence is proprietary)

Klon: DSG3-1535

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology (formalin) 1-2 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

A431 cells. Skin or Lung squamous cell carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Details zu Desmoglein 3

Target: Desmoglein 3 (DSG3)

Andere Bezeichnung: Desmoglein-3 (DSG3 Produkte)

Synonyme: DSG3 antikoerper, CDHF6 antikoerper, PVA antikoerper, bal antikoerper, balding antikoerper, desmoglein 3 antikoerper, DSG3 antikoerper, Dsg3 antikoerper

Hintergrund: 130  kDa pemphigus vulgaris antigen (PVA), Balding (Bal), Cadherin family member 6 (CDHF6), Desmoglein-3 (DSG3)

Molekulargewicht: 130 kDa

Gen-ID: 1830, 1925

UniProt: P32926