Desmoglein 1 Antikörper (CF®594)
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- Target Alle Desmoglein 1 (DSG1) Antikörper anzeigen
- Desmoglein 1 (DSG1)
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Reaktivität
- Human
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser Desmoglein 1 Antikörper ist konjugiert mit CF®594
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Applikation
- Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS)
- Verwendungszweck
- Mouse Monoclonal anti-Desmoglein-1 (DSG1/1733), CF594 Conjugate
- Produktmerkmale
- This antibody recognizes a protein of ~150 kDa, identified as Desmoglein-1 (DSG1). Desmoglein-1 is a member of the desmosomal cadherin family. Desmosomes are intercellular adhering junctions that represent cell surface attachment sites for intermediate filament. Desmocollins and desmogleins are the main desmosomal transmembrane proteins. Desmogleins consist of Dsg1, Dsg2, Dsg3, and Dsg4 isoforms. Within the desmosome, the extracellular domain of desmoglein is essential for calcium dependent heterophilic binding to the desmocollins, whereas the intracellular domain is essential for binding to the desmosomal plaque protein, plakoglobin. Desmoglein 1 is synthesized exclusively in the suprabasal layers. Intact and functionally active desmoglein 1 is essential to epidermal integrity. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogen
- Recombinant human desmoglein-1 protein fragment corresponding to intracellular domain (exact sequence is proprietary)
- Klon
- DSG1-1733
- Isotyp
- IgG1 kappa
- Top Product
- Discover our top product DSG1 Primärantikörper
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- Applikationshinweise
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Immunohistology (formalin) 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA pH 8.0 for 10-20 min followed by cooling at RT for 20 min
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 1-2 μg/mL
- Optimal dilution for a specific application should be determined by user
- Kommentare
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A431 or A-375 cells. Skin
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Protect from light
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- Target
- Desmoglein 1 (DSG1)
- Andere Bezeichnung
- Desmoglein-1 (DSG1 Produkte)
- Synonyme
- CDHF4 antikoerper, DG1 antikoerper, DSG antikoerper, PPKS1 antikoerper, SPPK1 antikoerper, DSG-1 antikoerper, DSG1 antikoerper, Dsg1b antikoerper, Dsg1c antikoerper, desmoglein 1 antikoerper, desmoglein 4 antikoerper, DSG1 antikoerper, DSG4 antikoerper, Dsg1 antikoerper
- Hintergrund
- Cadherin family member 4, CDHF4, Desmoglein-1, Desmosomal glycoprotein 1, DG1, DSG1, EPKHE, EPKHIA, Pemphigus foliaceus antigen, PPKS1, SPPK1
- Molekulargewicht
- 150 kDa (precursor), 160 kDa (mature)
- Gen-ID
- 1828, 2633
- UniProt
- Q02413
- Pathways
- Cell-Cell Junction Organization
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