Keratin 10/13 Antikörper
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- Target
- Keratin 10/13
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Reaktivität
- Human, Cat
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Unkonjugiert
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Applikation
- Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)
- Verwendungszweck
- Mouse Monoclonal anti-Cytokeratin 10/13 (DE-K13), Purified, BSA-free
- Produktmerkmale
- This antibody recognizes cytokeratin 10 (56.5 kDa) and cytokeratin 13 (53 kDa) in Western blotting. It recognizes only cytokeratin 13 in formalin-fixed, paraffin-embedded tissue sections. It does not react with cytokeratin 10 positive, cytokeratin 13 negative epithelia such as epidermis. However, on frozen sections this MAb serves as differentiation-related marker of all stratified epithelia, it stains all suprabasal cells in both cornifying and non-cornifying stratified epithelia and more differentiated cells of squamous carcinomas. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Aufreinigung
- Purified
- Immunogen
- Cytoskeletal preparation extracted from human ectocervical epithelium
- Klon
- DE-K13
- Isotyp
- IgG2a kappa
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- Applikationshinweise
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Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Western blotting 0.25-0.5 μg/mL
- Kommentare
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A431, HeLa, MCF7 cells. Tonsil, esophageal or bladder carcinoma.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 1 mg/mL
- Buffer
- PBS (no BSA, no azide)
- Konservierungsmittel
- Azide free
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- Target
- Keratin 10/13
- Andere Bezeichnung
- Cytokeratin 10/13
- Hintergrund
- BCIE, BIE, EHK, Keratin Type I Cytoskeletal 10, KRT10, CK13, K13, keratin type I cytoskeletal 13, Keratin-13, Krt1-13, KRT1
- Molekulargewicht
- 56.5 kDa (CK10) & 53 kDa (CK13)
- Gen-ID
- 3858, 99936
- UniProt
- P13645
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