UBE2F Antikörper (N-Term)

Produktnummer ABIN615974

Produktdetails anti-UBE2F Antikörper

Target: UBE2F (Ubiquitin-Conjugating Enzyme E2F (UBE2F))

Bindungsspezifität: N-Term

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Schwein

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser UBE2F Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequenz: SKLKRDDGLK GSRT-C

Spezifität: Recogfnizes NEDD8 conjugating enzyme (N Terminus).

Kreuzreaktivität (Details): Species reactivity (expected):Human, Mouse, Canine (Dog), Pig, Cow.
Species reactivity (tested):Rat.

Aufreinigung: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the N Terminus of the protein sequence according to NP_542409.1. Genename: UBE2F

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu UBE2F

Target: UBE2F (Ubiquitin-Conjugating Enzyme E2F (UBE2F))

Andere Bezeichnung: NCE2 (UBE2F Produkte)

Synonyme: NCE2 antikoerper, 2510010F15Rik antikoerper, AI851109 antikoerper, RGD1307608 antikoerper, zgc:77005 antikoerper, ubiquitin conjugating enzyme E2 F (putative) antikoerper, NEDD8-conjugating enzyme UBE2F antikoerper, ubiquitin-conjugating enzyme E2F (putative) antikoerper, ubiquitin conjugating enzyme E2F (putative) S homeolog antikoerper, UBE2F antikoerper, Tsp_01616 antikoerper, ube2f antikoerper, ube2f.S antikoerper, Ube2f antikoerper

Hintergrund: Ubiquitin is covalently attached to target proteins by a multienzymatic system consisting of E1 (ubiquitin-activating), E2 (ubiquitin-conjugating), and E3 (ubiquitin-ligating) enzymes. NEDD8, a ubiquitin-like protein, is conjugated to proteins in a manner analogous to ubiquitinylation. beta-amyloid precursor protein-binding protein-1 (APPBP1) can bind to NEDD8 in rabbit reticulocyte lysates. However, since APPBP1 shows similarity to only the N-terminal domain of an E1 enzyme, it must interact with a protein showing similarity to the C-terminal region of E1s. By searching sequence databases, a cDNAs encoding UBA3 was identified as the human homolog of yeast Uba3. The predicted 442-amino acid UBA3 protein shares 43 % sequence identity with yeast Uba3. In vitro, UBA3 formed a complex with APPBP1 and a thioester linkage with NEDD8. APPBP1/UBA3 complex may function as an E1-like enzyme for the activation of NEDD8.Synonyms: NEDD8-conjugating enzyme 2, UBE2F, Ubiquitin-conjugating enzyme E2 F

Gen-ID: 140739

NCBI Accession: NP_542409

UniProt: Q969M7