MILK FAT GLOBULIN Antikörper (CF®488A)

Produktnummer ABIN6156439

Produktdetails

Target: MILK FAT GLOBULIN

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: CF®488A

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Verwendungszweck: Mouse Monoclonal anti-Milk-Fat-Globulin (EDM45), CF488A Conjugate

Produktmerkmale: This antibody recognizes a protein of 40-45 kDa, identified as human milk fat globule membrane protein (HMFG). HMFG is present on normal human breast epithelial cells and cell lines derived from breast carcinomas, as well as to the outer surface of the human milk fat globule. HMFG is considered as a differentiation marker. It is useful as specific breast epithelial marker and can also provide a tool to study the role of the cell surface in normal and neoplastic mammary development. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Delipidated human milk fat globule membrane preparation

Klon: EDM45

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

Normal breast or breast carcinoma

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Antigendetails

Target: MILK FAT GLOBULIN

Andere Bezeichnung: Milk-Fat-Globulin

Hintergrund: Breast epithelial antigen BA46, EDIL1, HMFG, Lactadherin, Lactadherin precursor, Medin, MFGE8, MFGM, Milk fat globule-EGF factor 8 (MFG-E8)

Molekulargewicht: 45 kDa

Gen-ID: 4240, 3745

UniProt: Q08431