Hepatocyte Specific Antigen Antikörper (Biotin)

Produktnummer ABIN6152837

Produktdetails anti-Hepatocyte Specific Antigen Antikörper (Biotin)

Target: Hepatocyte Specific Antigen (HepPar-1)

Reaktivität: Human, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Hepatocyte Specific Antigen Antikörper ist konjugiert mit Biotin

Applikation: Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Verwendungszweck: Mouse Monoclonal anti-Hepatocyte-Specific (CPS1/1022), Biotin Conjugate

Produktmerkmale: This MAb recognizes a protein of 165 kDa, identified as carbamoyl phosphate synthetase 1 (CPS1). This mitochondrial enzyme catalyzes synthesis of carbamoyl phosphate from ammonia and bicarbonate. This reaction is the first committed step of the urea cycle, which is important in the removal of excess urea from cells.Deficiency of CPS1 is an autosomal recessive disorder that causes hyperammonemia. CPS1 is a hepatocyte specific protein that localizes to the mitochondria of hepatocytes. It is a sensitive marker for distinguishing hepatocellular carcinomas (HCC) from other metastatic carcinomas as well as cholangio-carcinomas. HCC s occur primarily in the stomach, but they are also found in many other organs. CPS1 may also be a useful marker for intestinal metaplasia. Reportedly, strong expression of CPS1 correlates with smaller tumor size and longer patient survival. Occasionally, CPS1 is also found in gastric carcinomas as well as in a few other non-hepatic tumors. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human CPS1 protein

Klon: CPS1-1022

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunohistology formalin-fixed 0.25-0.5 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1.0 μg/mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

HeLa cells. Liver or Hepatocellular Carcinoma (HCC)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Details zu Hepatocyte Specific Antigen

Target: Hepatocyte Specific Antigen (HepPar-1)

Andere Bezeichnung: Hepatocyte-Specific (HepPar-1 Produkte)

Hintergrund: Carbamoyl-phosphate synthetase 1 (CPS1), Carbamoylphosphate synthetase 1, CPSase 1, CPSASE1

Molekulargewicht: ~165 kDa

Gen-ID: 1373, 149252

UniProt: P31327