V5 Epitope Tag Antikörper (Biotin)

Produktnummer ABIN602522

Produktdetails anti-V5 Epitope Tag Antikörper (Biotin)

Target: V5 Epitope Tag

Reaktivität: Bitte anfragen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser V5 Epitope Tag Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (IHC)

Sequenz: GKPIPNPLLG LDST

Spezifität: Recognizes a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5. Has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with this epitope. Usually, a 14 amino acid tag has been added to the recombinant proteins, although a smaller epitope of 9 amino acids (that as a peptide inhibit the binding of the monoclonal antibody to its native protein) has also been successfully used. The 14 amino acid epitope is, gly lys pro ile pro asn pro leu leu gly leu asp ser thr. (The 9 amino acid epitope is underlined.

Aufreinigung: Protein G purified

Immunogen: Paramyxovirus SV5, Simian-Virus 5.

Type of Immunogen: Virus

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, IHC, IHC-Fr, WB (1:1000 - 1:5000)

Usage: Western Blot:1:1000-1:5000. ELISA. Immunohistochemistry (frozen). The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: IgG conjugated to Biotin. Supplied as a liquid, 0.1 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Aliquot to Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.

Details zu V5 Epitope Tag

Target: V5 Epitope Tag

Andere Bezeichnung: V5 Tag (V5 Epitope Tag Produkte)

Substanzklasse: Tag