Topo IIalpha Antikörper

Produktnummer ABIN5962665

Produktdetails

Target: Topo IIalpha

Reaktivität: Human, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Spezifität: Alpha isozyme,ATP hydrolyzing DNA topoisomerase II alfa,DNA gyrase,DNA topoisomerase (ATP hydrolyzing),DNA topoisomerase 2 alpha,DNA topoisomerase 2-alpha,DNA topoisomerase II 170 kD,DNA topoisomerase II alpha isozyme,DNA topoisomerase II,DNA Topoisomerase2,TOP 2A,TOP2,TOP2A,TOP2A,Topoisomerase DNA II alpha 170  kDa,TP2A

Aufreinigung: Affinity purification

Immunogen: Synthesized peptide derived from the N-terminal region of human Topo IIalpha

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:2000, IHC 1:100-1:300, IF 1:200-1:1000, ELISA 1:20000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % sodium azide,0.5 % BSA and 50 % glycerol  pH 7.4.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze / thaw cycles.

Antigendetails

Target: Topo IIalpha

Hintergrund: This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia.

Molekulargewicht: 174kDa

Gen-ID: 7153

UniProt: P11388