PCSK6 Antikörper (AA 637-648)

Produktnummer ABIN574302

Produktdetails anti-PCSK6 Antikörper

Target: PCSK6 (Proprotein Convertase Subtilisin/kexin Type 6 (PCSK6))

Bindungsspezifität: AA 637-648

Reaktivität: Human, Kaninchen

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser PCSK6 Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human PCSK6 / PACE4. This antibody is expected to recognize reported isoforms a, b, g and h (NP_002561.1, NP_612192.1, NP_612193.1, NP_612194.1 resp.).

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Rabbit (100%) Marmoset (92%) Mouse, Rat, Elephant, Panda, Bovine, Dog, Horse, Pig, Opossum (83%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-YHTFSAHQSRSR from an internal region of human PCSK6 / PACE4 (NP_002561.1, NP_612192.1, NP_612195.1, NP_612197.1, NP_612196.1, NP_612198.2, NP_612193.1, NP_612194.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Rabbit (100%), Marmoset (92%), Mouse, Rat, Elephant, Panda, Bovine, Dog, Horse, Pig, Opossum (83%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:32000)

Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Preliminary experiments gave an approx 40-45 kD band in Human Brain (Cerebellum) lysates after 0.2 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 106 kD according to NP_002561.1. The 40-45 kD band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported?

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu PCSK6

Target: PCSK6 (Proprotein Convertase Subtilisin/kexin Type 6 (PCSK6))

Andere Bezeichnung: PACE4 / PCSK6 (PCSK6 Produkte)

Synonyme: PACE4 antikoerper, PCSK6 antikoerper, SPC4 antikoerper, C86343 antikoerper, Pace4 antikoerper, Spc4 antikoerper, PACE4AIIa antikoerper, Xpace4 antikoerper, spc4 antikoerper, proprotein convertase subtilisin/kexin type 6 antikoerper, peptidase S8 antikoerper, proprotein convertase subtilisin/kexin type 6 L homeolog antikoerper, PCSK6 antikoerper, EAMY_RS27925 antikoerper, pcsk6 antikoerper, Pcsk6 antikoerper, pcsk6.L antikoerper

Hintergrund: Name/Gene ID: PCSK6
Subfamily: Serine S8
Family: Protease

Synonyms: PCSK6, PACE4, SPC4

Gen-ID: 5046

NCBI Accession: NP_002561, NP_612192, NP_612195, NP_612197, NP_612196, NP_612198, NP_612193, NP_612194

UniProt: P29122

Pathways: Neurotrophin Signalübertragung, SARS-CoV-2 Protein Interaktom