Kaninchen anti-Affe IgG (Heavy & Light Chain) Antikörper (Biotin) - Preadsorbed

Produktnummer ABIN5706891

Produktdetails

Target: IgG

Bindungsspezifität: Heavy & Light Chain

Reaktivität: Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Biotin

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Kreuzreaktivität: Affe

Produktmerkmale:

Synonyms: rabbit anti-Monkey IgG biotin conjugated antibody, rabbit anti-Monkey IgG antibody biotin conjugation

Background: Conjugated Anti-Monkey IgG (H&L) Biotin antibody generated in rabbit detects specifically monkey IgG heavy and light chains. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. This biotin conjugated anti-Monkey IgG (H&L) secondary antibody is ideal for investigators who routinely perform western blot, ELISA, Immunohistochemistry, and more general immunoassays. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment.

Aufreinigung: Preadsorption: immunoaffinity chromatography using Monkey IgG coupled to agarose beads

Immunogen:

Immunogen: Anti-Monkey IgG (H&L) was produced by repeated immunization with monkey IgG in rabbit.

Immunogen Type: Native Protein

Isotyp: IgG

Labeling Ratio: 10-20

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-Monkey IgG (H&L) Biotin conjugated Antibody is suitable for use in Western Blotting, Immunohistochemistry, ELISA as well as other antibody detection methods. Specific conditions for reactivity and signal detection should be optimized by the end user.

ELISA Dilution: 1:50,000 - 1:100,000

Western Blot Dilution: 1:5,000 - 1:10,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: RT,4 °C,-20 °C

Haltbarkeit: 12 months

Antigendetails

Target: IgG

Abstract: IgG Produkte

Substanzklasse: Antibody

Hintergrund:

Synonyms: rabbit anti-Monkey IgG biotin conjugated antibody, rabbit anti-Monkey IgG antibody biotin conjugation

Background: Conjugated Anti-Monkey IgG (H&L) Biotin antibody generated in rabbit detects specifically monkey IgG heavy and light chains. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. This biotin conjugated anti-Monkey IgG (H&L) secondary antibody is ideal for investigators who routinely perform western blot, ELISA, Immunohistochemistry, and more general immunoassays. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment.