Histone H4 Antikörper (acLys8)
-
- Target Alle Histone H4 Antikörper anzeigen
- Histone H4
-
Bindungsspezifität
- acLys8
-
Reaktivität
- Human, Maus, Ratte, Huhn, Xenopus laevis, Drosophila melanogaster, C. elegans, Pflanzen
-
Wirt
- Kaninchen
-
Klonalität
- Polyklonal
-
Konjugat
- Dieser Histone H4 Antikörper ist unkonjugiert
-
Applikation
- Western Blotting (WB), Immunohistochemistry (IHC), Chromatin Immunoprecipitation (ChIP), Fluorescence Microscopy (FM)
- Produktmerkmale
-
Synonyms: rabbit anti-Histone H4 Ac Lys8 antibody, H4K8ac, HIST2H4B, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4, HIST2H4A, HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, histone cluster 4, H4, histone 4, H4
Background: Chromatin is the arrangement of DNA and proteins in which chromosomes are formed. Correspondingly, chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is a very dynamic structure in which numerous post-translational modifications work together to activate or repress the availability of DNA to be copied, transcribed, or repaired. These marks decide which DNA will be open and commonly active (euchromatin) or tightly wound to prevent access and activation (heterochromatin). Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. In particular, acetylation of H4 Lys5 (H4 Lys5Ac) has been linked to transcriptional activation and DNA repair. Newly assembled histones are typically acetylated on H4 at lysine 5 and 12. The enzyme histone acetyltransferase 1 (HAT1) is the primary modulator, and these marks are necessary for complete chromatin assembly. Anti-Histone H4 are ideal for researchers interested in Chromatin Research, DNA replication Transcription Translation and Splicing, Epigenetics, Chromatin Modifiers, Histones and Modified Histones, DNA Repair, and HA Epitope Tags research.
Gene Name: HIST4H4
- Aufreinigung
- Anti-Histone H4 [ac Lys8] was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H4. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Cross-reactivity with Histone H4 from other sources has not been determined.
- Immunogen
-
Immunogen: Histone H4 [ac Lys8] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic aceylated peptide surrounding Lysine 8 of human Histone H4.
Immunogen Type: Peptide
- Isotyp
- IgG
- Top Product
- Discover our top product Histone H4 Primärantikörper
-
-
- Applikationshinweise
-
Immunohistochemistry Dilution: 1:1000-1:2000
Application Note: Anti-Histone H4 [ac Lys8] antibody is useful for Western Blot, Dot Blot, Chromatin Immunoprecipitation, and Immunofluorescence/Immunocytochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~13 kDa corresponding to Histone H4 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.
ChIP Dilution: 2-5 μg/million cells
Western Blot Dilution: 0.2 μg/mL
IF Microscopy Dilution: 1:1000-1:2000
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Format
- Liquid
- Konzentration
- 0.85 mg/mL
- Buffer
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 30 % Glycerol
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Haltbarkeit
- 12 months
-
- Target
- Histone H4
- Abstract
- Histone H4 Produkte
- Synonyme
- hist1h4l antikoerper, H1ft antikoerper, Hist4 antikoerper, Hist4h4 antikoerper, H4 antikoerper, X04652 antikoerper, FO108 antikoerper, H4/n antikoerper, H4F2 antikoerper, H4FN antikoerper, HIST2H4 antikoerper, H4C13 antikoerper, H4C7 antikoerper, F8N16.2 antikoerper, F8N16_2 antikoerper, histone H4 antikoerper, BcDNA:RH52884 antikoerper, CG3379 antikoerper, Dmel\CG3379 antikoerper, H4r antikoerper, His4-88CD antikoerper, His4R antikoerper, histone cluster 1, H4a L homeolog antikoerper, histone cluster 1 H4 family member B antikoerper, histone cluster 2, H4 antikoerper, histone cluster 2 H4 family member a antikoerper, histone H4 antikoerper, Histone H4 replacement antikoerper, Histone H4 antikoerper, hist1h4a.L antikoerper, Hist1h4b antikoerper, Hist2h4 antikoerper, HIST2H4A antikoerper, H4 antikoerper, H4C14 antikoerper, HIS4 antikoerper, HIST2H4 antikoerper, His4r antikoerper, his-38 antikoerper
- Gen-ID
- 121504
- NCBI Accession
- NP_001029249
- UniProt
- P62805
-