Histone 3 Antikörper (2meLys18)
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- Target Alle Histone 3 (H3) Antikörper anzeigen
- Histone 3 (H3)
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Bindungsspezifität
- 2meLys18
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Reaktivität
- Human, Maus, Ratte, Huhn, Xenopus laevis, C. elegans, Drosophila melanogaster, Pflanzen
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser Histone 3 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunohistochemistry (IHC), Chromatin Immunoprecipitation (ChIP), Fluorescence Microscopy (FM)
- Produktmerkmale
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Synonyms: rabbit anti-Histone H3 dimethyl Lys18 antibody, H3.3AH3F3H3F3B, H3.3B, H3 histone, family 3A, histone H3.3, MGC87783, MGC87782, H3K18me2
Background: The di-methylated K18 on histone H3 is a seemingly transient post-translational modification. H3K18 is better known to be acetylated, and occasionally mono-methylated. Suv39h1, a well-studied histone methyltransferase seems to be responsible for the transition of acetylation and methylation at this H3 modification site. The di-methylated K18 on H3 seems to be associated with embryological development and possibly implantation. Anti-Histone H3 are ideal for researchers interested in Chromatin Modifiers, Chromatin Research, Histones and Modified Histones, and Epigenetics research.
Gene Name: HIST2H3C
- Aufreinigung
- Anti-Histone H3 [Dimethyl Lys18] was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H3.2. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.
- Immunogen
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Immunogen: Histone H3 [Dimethyl Lys18] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic dimethylated peptide surrounding Lysine 18 of human Histone H3.2.
Immunogen Type: Peptide
- Isotyp
- IgG
- Top Product
- Discover our top product H3 Primärantikörper
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- Applikationshinweise
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Immunohistochemistry Dilution: 1:50
Application Note: Anti-Histone H3 [Dimethyl Lys18] antibody is useful for Western Blot, Chromatin Immunoprecipitation, Dot Blot, Immunocytochemistry, and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.
ChIP Dilution: 2-5 μg/million cells
Western Blot Dilution: 1:500
IF Microscopy Dilution: 1:50
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 0.79 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 30 % Glycerol
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Haltbarkeit
- 12 months
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- Target
- Histone 3 (H3)
- Andere Bezeichnung
- Histone H3 (H3 Produkte)
- Synonyme
- H-3 antikoerper, histocompatibility 3 antikoerper, H3 antikoerper
- Gen-ID
- 126961
- NCBI Accession
- NP_001005464
- UniProt
- Q71DI3
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