NUDT16 Antikörper

Produktnummer ABIN5705803

Produktdetails anti-NUDT16 Antikörper

Target: NUDT16 (Nudix (Nucleoside Diphosphate Linked Moiety X)-Type Motif 16 (NUDT16))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NUDT16 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Immunogen: nudix (nucleoside diphosphate linked moiety X)-type motif 16

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

HeLa cells were subjected to SDS PAGE followed by western blot with FNab05901(NUDT16 antibody) at dilution of 1:2500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol  pH 7.3

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze / thaw cycles.

Lagerung: -20 °C

Haltbarkeit: 12 months

Details zu NUDT16

Target: NUDT16 (Nudix (Nucleoside Diphosphate Linked Moiety X)-Type Motif 16 (NUDT16))

Andere Bezeichnung: NUDT16 (NUDT16 Produkte)

Synonyme: RGD1311387 antikoerper, 2310041H06Rik antikoerper, 2810047L04Rik antikoerper, 2900006H04Rik antikoerper, AI851783 antikoerper, x29 antikoerper, nudix hydrolase 16 antikoerper, nudix (nucleoside diphosphate linked moiety X)-type motif 16 antikoerper, nudix hydrolase 16 L homeolog antikoerper, Nudt16 antikoerper, NUDT16 antikoerper, nudt16.L antikoerper

Hintergrund: Synonyms:Nudix motif 16, NUDT16, U8 snoRNA binding protein H29K, U8 snoRNA decapping enzyme Background:RNA-binding and decapping enzyme that catalyzes the cleavage of the cap structure of snoRNAs and mRNAs in a metal-dependent manner. Part of the U8 snoRNP complex that is required for the accumulation of mature 5.8S and 28S rRNA. Has diphosphatase activity and removes m7G and/or m227G caps from U8 snoRNA and leaves a 5'monophosphate on the RNA. Catalyzes also the cleavage of the cap structure on mRNAs. Does not hydrolyze cap analog structures like 7-methylguanosine nucleoside triphosphate(m7GpppG). Also hydrolysis m7G-and m227G U3-capped RNAs but with less efficiencies. Has broad substrate specificity with manganese or cobalt as cofactor and can act on various RNA species. Binds to the U8 snoRNA, metal is not required for RNA-binding. May play a role in the regulation of snoRNAs and mRNAs degradation. Acts also as a phosphatase, hydrolyzes the non-canonical purine nucleotides inosine diphosphate(IDP) and deoxyinosine diphosphate(dITP) as well as guanosine diphosphate(GDP), deoxyguanosine diphosphate(dGDP), xanthine diphosphate(XDP), inosine triphosphate(ITP) and deoxyinosine triphosphate(ITP) to their respective monophosphate derivatives and does not distinguish between the deoxy-and ribose forms(PubMed:20385596, PubMed:26121039). The order of activity with different substrates is IDP > dIDP >> GDP = dGDP > XDP = ITP = dITP(PubMed:20385596). Binds strongly to GTP, ITP and XTP. Participates in the hydrolysis of dIDP/IDP and probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions(PubMed:20385596).

Molekulargewicht: 21 kDa

Gen-ID: 131870

UniProt: Q96DE0

Pathways: Positive Regulation of Response to DNA Damage Stimulus