PAF1/PD2 Antikörper

Produktnummer ABIN5704209

Produktdetails anti-PAF1/PD2 Antikörper

Target: PAF1/PD2 (PAF1) (Paf1, RNA Polymerase II Associated Factor, Homolog (PAF1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PAF1/PD2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Immunogen: Paf1, RNA polymerase II associated factor, homolog (S. cerevisiae)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

HepG2 cells were subjected to SDS PAGE followed by western blot with FNab06106(PAF1 antibody) at dilution of 1:1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol  pH 7.3

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze / thaw cycles.

Lagerung: -20 °C

Haltbarkeit: 12 months

Details zu PAF1/PD2

Target: PAF1/PD2 (PAF1) (Paf1, RNA Polymerase II Associated Factor, Homolog (PAF1))

Andere Bezeichnung: PAF1 (PAF1 Produkte)

Synonyme: F23149_1 antikoerper, PD2 antikoerper, 5730511K23Rik antikoerper, AA675045 antikoerper, f23149_1 antikoerper, pd2 antikoerper, im:6906070 antikoerper, paf1l antikoerper, wu:fb80a09 antikoerper, paf1 antikoerper, RGD1306219 antikoerper, PAF1 homolog, Paf1/RNA polymerase II complex component antikoerper, Paf1, RNA polymerase II complex component antikoerper, PAF1 homolog, Paf1/RNA polymerase II complex component L homeolog antikoerper, RNA polymerase II-associated factor 1 homolog pseudogene antikoerper, RNA polymerase II-associated factor 1 homolog antikoerper, paf1, RNA polymerase II associated factor, homolog (S. cerevisiae) antikoerper, Paf1, RNA polymerase II associated factor, homolog (S. cerevisiae) antikoerper, PAF1 antikoerper, Paf1 antikoerper, paf1 antikoerper, paf1.L antikoerper, LOC100019208 antikoerper, LOC100161171 antikoerper, LOC100639019 antikoerper, LOC100650306 antikoerper

Hintergrund: Synonyms:PD2 Background:Component of the PAF1 complex(PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'-and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1, it promotes leukemogenesis through association with KMT2A/MLL1-rearranged oncoproteins, such as KMT2A/MLL1-MLLT3/AF9 and KMT2A/MLL1-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4'(H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B(H2BK120ub1), UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Connects PAF1C with the RNF20/40 E3 ubiquitin-protein ligase complex. Involved in polyadenylation of mRNA precursors. Has oncogenic activity in vivo and in vitro.

Gen-ID: 54623

UniProt: Q8N7H5

Pathways: Cellular Response to Molecule of Bacterial Origin, Stem Cell Maintenance