Schwein anti-Maus IgG (Heavy & Light Chain) Antikörper
IP
Polyclonal
unconjugated
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- Target Alle IgG Produkte
- IgG
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Bindungsspezifität
- Heavy & Light Chain
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Reaktivität
- Maus
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Wirt
- Schwein
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Klonalität
- Polyklonal
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Applikation
- Immunoprecipitation (IP)
- Spezifität
- Precipitating polyclonal Swine antiSerum to Mouse IgG, heavy and light chains.
- Kreuzreaktivität (Details)
- Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
- Produktmerkmale
- The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in mouse serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In immunoelectrophoresis and double radial immunodiffusion to identify the presence of IgG, as secondary antibody to precipitate the immunoglobulin in normal human serum, as bridging reagent in a peroxidase antiperoxidase technique using M/PAP, to prepare an immunoadsorbent for the production of Ig-free mouse serum, plasma or non-Ig preparations.
- Aufreinigung
- Purified
- Immunogen
- Purified normal IgG isolated from pooled mouse serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
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Goat anti-Mouse IgG (Heavy & Light Chain) antibody - Preadsorbed
ELISA, IHC, WB, FACS, FLISA, FM, IP, LF Polyclonal IgG unconjugated
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- Applikationshinweise
- In immunoelectrophoresis use 2 μL or equivalent against 120 μL antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μL antiserum in a 3 mm diameter centre well and 2 μL serum samples (neat and diluted) in 2 mm diameter peripheral wells.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Buffer
- Purified hyperimmune swine IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added.
- Konservierungsmittel
- Without preservative
- Lagerung
- 4 °C/-20 °C
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- Target
- IgG
- Abstract
- IgG Produkte
- Substanzklasse
- Antibody
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