TNFSF13 Antikörper (AA 105-250)

Produktnummer ABIN5690647

Produktdetails anti-TNFSF13 Antikörper

Target: TNFSF13 (Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13))

Bindungsspezifität: AA 105-250

Reaktivität: Human, Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser TNFSF13 Antikörper ist unkonjugiert

Applikation: ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC)

Aufreinigung: APRIL (monoclonal Sacha-2) antibody was purified from concentrated hybridoma tissue culture supernatant. APRIL (monoclonal Sacha-2) antibody was purified to 95% in SDS-PAGE.

Immunogen: APRIL (monoclonal Sacha-2) antibody was raised against recombinant human APRIL (aa. 105-250).

Klon: Sacha-2

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: APRIL antibody can be used to recognize human and mouse APRIL by ELISA (capture), Flow Cytometry, and Immunocytochemistry. Method: HEK-293T cells expressing surface membrane associated human APRIL (aa 92-233) or a mock control were injected in chicken liver and frozen in OCT. 8 μ,m sections of injected frozen liver were fixed in acetone, washed in PBS and incubated with Sacha-2 at 10 μ,g/mL for 1hr after blocking in PBS/1 % BSA. Human APRIL was revealed with 3-amino-9-Ethyl-carbazole (AEC) following 45 min. incubation with goat anti-rat HRP. Method: HEK 293T  cells (5 x 105) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of mouse APRIL (thick line). Cells were incubated on ice for 30 min in 50 μ,l FACS buffer (PBS, 5 % Fetal calf serum, 0.02 % sodium azide) containing 1 μ,g/mL of Sacha-2 antibody.  After washing in FACS buffer, PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and then analyzed by flow cytometry. This product is for research use only.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS containing 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: APRIL antibody can be stored at 4°C, stable for one year.

Details zu TNFSF13

Target: TNFSF13 (Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13))

Andere Bezeichnung: APRIL (TNFSF13 Produkte)

Synonyme: APRIL antikoerper, CD256 antikoerper, TALL-2 antikoerper, TALL2 antikoerper, TRDL-1 antikoerper, ZTNF2 antikoerper, 2310026N09Rik antikoerper, April antikoerper, Tall2 antikoerper, Trdl1 antikoerper, SLAN antikoerper, ANP32B antikoerper, april antikoerper, tnf13 antikoerper, TNLG7B antikoerper, TNF superfamily member 13 antikoerper, tumor necrosis factor (ligand) superfamily, member 13 antikoerper, acidic leucine-rich nuclear phosphoprotein 32 family member B antikoerper, TNFSF13 antikoerper, Tnfsf13 antikoerper, LOC494442 antikoerper, tnfsf13 antikoerper

Gen-ID: 8741, 9370

UniProt: Q15848

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response