RAF1 Antikörper (pSer301)

Produktnummer ABIN5688894

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch RAF1 in WB. Es zeigt Reaktivität gegenüber Proben von Human, Maus, Ratte, Rind (Kuh), Huhn, Hund und Xenopus laevis.

Kurzübersicht für RAF1 Antikörper (pSer301) (ABIN5688894)

Target: RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Huhn, Hund, Xenopus laevis

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RAF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-RAF1 Antikörper

Bindungsspezifität: pSer301

Aufreinigung: Affinity Purified

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser301 of rat Raf-1.

Anwendungsinformationen

Applikationshinweise: The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, chicken, human, mouse, non-human primate and Xenopus based on the fact that these species have 100 % homology with the amino acid sequence used as antigen.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 μLin 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per mL BSA and 50 % glycerol.

Lagerung: -20 °C

Informationen zur Lagerung: Raf-1 antibody can be stored at -20°C and is stable at -20°C for at least 1 year.

Details zu RAF1

Target: RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))

Andere Bezeichnung: Raf-1

Hintergrund: The Ras pathway is a critical signal transduction cascade involved in regulating cellular proliferation, differentiation, survival, and oncogenic transformation. Members of the Raf serine/threonine kinase family are key intermediates in this cascade, functioning to relay signals from activated Ras to the downstream protein kinases MEK and ERK (Marshall, 1996). Previous studies have shown that phosphorylation is required for Raf-1 activation (Dhillon and Kolch, 2002, Chong et al., 2003). Recent work has demonstrated that phosphorylation also regulates the downregulation of Raf (Dougherty et al., 2005) with two sites participating: Ser301 and Ser642.

Molekulargewicht: 74 kDa

Gen-ID: 24703

UniProt: P11345

Pathways: MAPK Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagie, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling, BCR Signaling