Crotonaldehyde (CRA) Antikörper (HRP)
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- Target
- Crotonaldehyde (CRA)
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- HRP
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Applikation
- Western Blotting (WB), ELISA
- Spezifität
- Specific for Crotonaldehyde modified proteins. Does not cross-react with Acrolein, Hexanoyl Lysine, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, Malondialdehyde, or Methylglyoxal modified proteins.
- Aufreinigung
- Protein G Purified
- Immunogen
- Crotonaldehyde modified Keyhole Limpet Hemocyanin (KLH)
- Klon
- 2A8-1
- Isotyp
- IgG1
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- Applikationshinweise
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- WB (1:1000)
- ELISA (1:1000)
- optimal dilutions for assays should be determined by the user.
- Kommentare
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A 1:1000 dilution of ABIN5650478 was sufficient for detection of Crotonaldehyde-modified proteins in 1 μg of Crotonaldehyde-BSA conjugate by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 1 mg/mL
- Buffer
- PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C
- Informationen zur Lagerung
- Conjugated antibodies should be stored at 4°C
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- Target
- Crotonaldehyde (CRA)
- Andere Bezeichnung
- Crotonaldehyde
- Substanzklasse
- Chemical
- Hintergrund
- Lipid peroxidation occurs when oxidizing agents attack carbon-carbon double bonds found in unsaturated lipids. In addition to membrane degradation, oxidation end-products have been found to damage cell viability through their mutagenic and toxic properties. These downstream functional consequences facilitate the development of disease and premature aging. Crotonaldehyde (CRA) is a carcinogenic, conjugated aldehyde produced endogenously via lipid peroxidation. It covalently modifies biomolecules, forming adducts with DNA and nucleophilic amino acid residues of proteins such as lysine (1).
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