Sucrose Phosphorylase Antikörper (HRP)

Produktnummer ABIN5596915

Produktdetails anti-Sucrose Phosphorylase Antikörper (HRP)

Target: Sucrose Phosphorylase

Reaktivität: E. coli

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Sucrose Phosphorylase Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), Immunoprecipitation (IP), ELISA

Kreuzreaktivität (Details): Cross reactivity against Sucrose Phosphorylase from other sources is unknown.

Aufreinigung: Sucrose Phosphorylase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Sucrose Phosphorylase [E.coli].

Immunogen:

Immunogen: Sucrose Phosphorylase [E.coli]

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-Sucrose Phosphorylase has been assayed against 1.0 μg of Sucrose Phosphorylase [E.coli] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Sucrose Phosphorylase

Target: Sucrose Phosphorylase

Abstract: Sucrose Phosphorylase Produkte

Synonyme: sucrose phosphorylase antikoerper, Sucrose phosphorylase antikoerper, RB12343 antikoerper, Shewmr4_0064 antikoerper, Shewmr7_0062 antikoerper, Sfri_3990 antikoerper, Acid_2070 antikoerper, Shewana3_0066 antikoerper, BAD_RS00415 antikoerper, Pnap_0232 antikoerper, Shew185_0061 antikoerper, Blon_0128 antikoerper, Lm4b_00279 antikoerper, Apre_0241 antikoerper, EAMY_RS33260 antikoerper, Spirs_0911 antikoerper, SpiBuddy_2458 antikoerper, Lbuc_1855 antikoerper

Hintergrund:

Synonyms: Putative sucrose phosphorylase Sucrose glucosyltransferase

Background: Sucrose Phosphorylase is an important enzyme in the metabolism of sucrose and regulation of other metabolic intermediates. Sucrose phosphorylase is in the class of hexosyltransferases. It has been shown in multiple experiments that the enzyme catalyzes this conversion by a double displacement mechanism.

Gene Name: ycjM

Gen-ID: 4492194

UniProt: P76041